The ACOT8 antibody is a research tool designed to detect and study Acyl-CoA thioesterase 8 (ACOT8), an enzyme involved in lipid metabolism. ACOT8. also known as thioesterase superfamily member 2 (THEM2), belongs to the acyl-CoA thioesterase family, which catalyzes the hydrolysis of acyl-CoA thioesters to free fatty acids and coenzyme A. This enzymatic activity regulates cellular levels of acyl-CoA substrates, influencing lipid synthesis, energy metabolism, and signaling pathways. ACOT8 is localized in mitochondria and peroxisomes, with roles in maintaining lipid homeostasis and modulating metabolic stress responses.
Research links ACOT8 to various physiological and pathological processes, including insulin resistance, cancer progression, and neurodegenerative diseases. For instance, ACOT8 overexpression has been associated with altered fatty acid oxidation in metabolic disorders. Antibodies against ACOT8 enable detection via techniques like Western blotting, immunohistochemistry, and immunofluorescence, aiding in protein expression profiling, subcellular localization studies, and mechanistic investigations. Commercial ACOT8 antibodies are typically raised in rabbits or mice, targeting specific epitopes within the protein. Validation includes testing for specificity, sensitivity, and cross-reactivity. These tools are essential for exploring ACOT8's biological functions and its potential as a therapeutic target in metabolic and oncological research.