ALPK1 (Alpha-protein kinase 1) is a serine/threonine kinase implicated in innate immune signaling and inflammatory pathways. It gained attention for its role in detecting bacterial metabolites, particularly ADP-L-glycero-β-D-manno-heptose (ADP-heptose), a pathogen-associated molecular pattern (PAMP) derived from Gram-negative bacteria. ALPK1 activation triggers the TIFA/TRAF6 signaling cascade, leading to NF-κB activation and pro-inflammatory cytokine production. This mechanism links ALPK1 to host defense against bacterial infections and chronic inflammation. Dysregulation of ALPK1 has been associated with inflammatory disorders, including gout, where hyperactivation may drive monosodium urate crystal-induced inflammation. ALPK1 antibodies are essential tools for studying its expression, phosphorylation status, and interaction partners in immune cells or tissues. Monoclonal and polyclonal antibodies targeting specific ALPK1 epitopes are used in techniques like Western blotting, immunofluorescence, and ELISA to elucidate its biological functions. Recent studies also explore ALPK1's potential involvement in cancer progression and autoimmune diseases, highlighting its dual role in infection response and pathological inflammation. Developing selective ALPK1 inhibitors or modulators is an emerging therapeutic strategy, necessitating reliable antibodies for preclinical validation.