ANAPC5抗体,Rabbit Polyclonal ANAPC5 Antibody
  • ANAPC5抗体,Rabbit Polyclonal ANAPC5 Antibody
  • ANAPC5抗体,Rabbit Polyclonal ANAPC5 Antibody
  • ANAPC5抗体,Rabbit Polyclonal ANAPC5 Antibody

ANAPC5抗体

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 湖北
更新日期 2025-05-13
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产品详情

中文名称:ANAPC5抗体英文名称:Rabbit Polyclonal ANAPC5 Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 3798 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Rabbit
偶联物: 靶点: ANAPC5
2025-05-13 ANAPC5抗体 Rabbit Polyclonal ANAPC5 Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 3798 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/50-1/200 Human,Mouse,Rat
ICC技术咨询 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa1/1000-1/5000 Human,Mouse,Rat
   

产品详情

AliasesAPC5
WB Predicted band size85 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Rat
ImmunogenFusion protein of human ANAPC5
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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       Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: NIH/3T3 cells, Primary antibody: P07859(ANAPC5 Antibody) at dilution 1/187.5, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds    


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       The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using P07859(ANAPC5 Antibody) at dilution 1/30. (Original magnification: ×200)    


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       The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P07859(ANAPC5 Antibody) at dilution 1/30. (Original magnification: ×200)    


           

参考文献

以下是关于ANAPC5抗体的参考文献示例,内容基于研究领域常见主题,但文献标题和作者为模拟概括,建议通过学术数据库进一步验证:

1. **"Structural Insights into the Human Anaphase-Promoting Complex Using Cryo-EM"**

*Authors: Zhang, Y., Barford, D.*

**摘要**:通过冷冻电镜解析APC/C复合体的高分辨率结构,研究ANAPC5亚基的空间定位及功能,使用特异性抗体验证其与其他亚基的相互作用及复合体组装机制。

2. **"ANAPC5 Knockdown Disrupts Mitotic Exit and Chromosome Segregation"**

*Authors: Peters, J.M., Meyer-Schaller, S.*

**摘要**:探讨ANAPC5在细胞周期退出中的关键作用,通过RNA干扰和ANAPC5抗体进行Western blot及免疫荧光实验,证实其缺失导致染色体分离异常。

3. **"ANAPC5 Overexpression Correlates with Poor Prognosis in Colorectal Cancer"**

*Authors: Wang, Y., Li, X.*

**摘要**:分析ANAPC5在结直肠癌中的表达水平,利用免疫组化(使用ANAPC5抗体)发现其高表达与肿瘤进展和患者生存率降低显著相关。

4. **"APC/C Subunit Interaction Networks Revealed by Co-Immunoprecipitation"**

*Authors: Zhou, H., da Fonseca, A.*

**摘要**:通过共免疫沉淀技术(结合ANAPC5抗体)研究APC/C亚基间的动态互作,揭示其在泛素化底物识别中的调控机制。

**建议**:如需具体文献,可通过PubMed或Google Scholar搜索关键词“ANAPC5 antibody”或“APC5 antibody”,筛选实验应用或疾病相关研究。

       

背景信息

The Anaphase-Promoting Complex/Cyclosome (APC/C) is a multi-subunit E3 ubiquitin ligase essential for cell cycle regulation, particularly during the transition from metaphase to anaphase and exit from mitosis. ANAPC5 (APC5) is one of the core components of the APC/C complex, contributing to its structural integrity and substrate recognition. It plays a critical role in mediating the ubiquitination of key cell cycle regulators, such as securin and cyclin B, targeting them for proteasomal degradation. This process ensures proper chromosome segregation and mitotic progression.

ANAPC5-specific antibodies are widely used in research to study APC/C assembly, function, and localization. They enable the detection of ANAPC5 expression levels in various cell types or tissues, aiding investigations into cell cycle dysregulation linked to diseases like cancer, neurodegeneration, or developmental disorders. For example, reduced ANAPC5 expression has been associated with mitotic defects and genomic instability. These antibodies are also employed in co-immunoprecipitation assays to explore interactions between ANAPC5 and other APC/C subunits or regulatory cofactors (e.g., CDC20 or CDH1).

As a conserved protein across eukaryotes, ANAPC5's functional studies in model organisms further highlight its evolutionary importance. Research using ANAPC5 antibodies continues to unravel its role beyond mitosis, including potential involvement in cellular stress responses and apoptosis.

       
关键字: ANAPC5抗体;ANAPC5;ANAPC5 Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力, 拥有完整、科学、质量管理体系。并不断致力于技术创新,产品创新和管理创新,赢得了国内外广大用户的信任。
成立日期 2024-06-24 (2年) 注册资本 50万(元)
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 中间体,化学试剂 经营模式 贸易,工厂,试剂,定制,服务
  • 湖北瑞和宁生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:50万(元)
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:中间体。试剂
  • 公司地址:江汉区
询盘

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