The CEP97 antibody is a research tool used to detect and study the centrosomal protein CEP97. a key component of the centrosome—a microtubule-organizing center critical for cell division, cytoskeletal organization, and cilia formation. CEP97 interacts with proteins like CEP161 and is implicated in regulating centriole duplication, ciliary disassembly, and cell cycle progression. It plays a role in suppressing spontaneous ciliogenesis by stabilizing the mother centriole and controlling microtubule anchoring. Dysregulation of CEP97 has been linked to ciliopathies, developmental disorders, and cancer, where centrosomal abnormalities are common.
The antibody is widely used in immunofluorescence, Western blotting, and immunoprecipitation to localize CEP97 within cells, assess its expression levels, or study its interactions in pathways like the DNA damage response. Researchers employ it to explore mechanisms underlying centrosome amplification, ciliary defects, or mitotic errors. Commercial CEP97 antibodies are often validated for specificity using knockout cell lines or siRNA knockdown.
Studies involving CEP97 contribute to understanding cellular architecture, signaling pathways, and disease mechanisms, particularly in contexts where centrosomal dysfunction drives pathology. Its role in ciliary resorption also links it to sensory and developmental processes, making it relevant in neurobiology and oncology research.