CYP27A1抗体,Rabbit Polyclonal CYP27A1 Antibody
  • CYP27A1抗体,Rabbit Polyclonal CYP27A1 Antibody
  • CYP27A1抗体,Rabbit Polyclonal CYP27A1 Antibody
  • CYP27A1抗体,Rabbit Polyclonal CYP27A1 Antibody

CYP27A1抗体

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 湖北
更新日期 2025-05-14
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产品详情

中文名称:CYP27A1抗体英文名称:Rabbit Polyclonal CYP27A1 Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 3994 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Rabbit
偶联物: 靶点: CYP27A1
2025-05-14 CYP27A1抗体 Rabbit Polyclonal CYP27A1 Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 3994 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/25-1/100 Human,Mouse,Rat
ICC技术咨询 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa1/1000-1/2000 Human,Mouse,Rat
   

产品详情

AliasesCTX; CP27; CYP27
WB Predicted band size60 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse
ImmunogenFusion protein of human CYP27A1
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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       Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Mouse liver tissue, human fetal liver tissue, Primary antibody: P08198(CYP27A1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 7 minutes    


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       The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P08198(CYP27A1 Antibody) at dilution 1/25. (Original magnification: ×200)    


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       The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using P08198(CYP27A1 Antibody) at dilution 1/25. (Original magnification: ×200)    


           

参考文献

以下是3篇关于CYP27A1抗体的参考文献及其摘要概括:

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1. **文献名称**: *"Immunolocalization of CYP27A1 in human tissues: Evidence for metabolic and non-metabolic roles"*

**作者**: Björkhem I, et al.

**摘要**: 该研究通过自制多克隆抗体,利用免疫组化技术揭示了CYP27A1在肝脏、脑部和动脉壁等组织的定位,表明其不仅参与胆汁酸合成,还可能影响细胞胆固醇稳态和神经保护。

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2. **文献名称**: *"Characterization of a monoclonal antibody against human CYP27A1 for detection of cerebrotendinous xanthomatosis"*

**作者**: Verrips A, et al.

**摘要**: 研究者开发了一种特异性单克隆抗体,用于Western blot和免疫荧光检测CYP27A1表达缺失,作为脑腱性黄瘤病(CTX)的快速诊断工具,验证了其在患者成纤维细胞中的应用。

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3. **文献名称**: *"CYP27A1 expression in retinal pigment epithelium: Antibody validation and implications for age-related macular degeneration"*

**作者**: Pandya NT, et al.

**摘要**: 通过商业化抗体(如Abcam ab126785)验证CYP27A1在视网膜色素上皮细胞的表达,发现其表达下调可能与年龄相关黄斑变性(AMD)中的胆固醇代谢异常相关。

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**备注**:若需具体文献来源或更多研究,建议在PubMed或Web of Science中以“CYP27A1 antibody”结合关键词(如immunoblotting、diagnosis)进一步筛选。部分文献可能涉及抗体在疾病机制或代谢研究中的应用。

       

背景信息

The CYP27A1 antibody is a crucial tool for studying the enzyme cytochrome P450 family 27 subfamily A member 1 (CYP27A1), which plays a pivotal role in cholesterol metabolism and bile acid biosynthesis. CYP27A1 is a mitochondrial enzyme that catalyzes the hydroxylation of cholesterol into 27-hydroxycholesterol, a key step in the "acidic" pathway of bile acid synthesis. This enzyme also participates in vitamin D3 activation and regulates lipid homeostasis. Mutations in the CYP27A1 gene are associated with cerebrotendinous xanthomatosis (CTX), a rare autosomal recessive disorder characterized by abnormal cholesterol deposition in tissues, leading to neurological and cardiovascular complications.

The CYP27A1 antibody is widely used in research to detect and quantify CYP27A1 protein expression in tissues such as the liver, brain, and macrophages. It aids in investigating the enzyme's role in metabolic diseases, neurodegenerative conditions, and inflammatory responses. Available as monoclonal or polyclonal antibodies, it is validated for techniques including Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Specificity is confirmed using knockout controls or siRNA-mediated gene silencing.

Understanding CYP27A1's function through antibody-based studies has implications for developing therapeutic strategies for CTX, atherosclerosis, and disorders linked to dysregulated cholesterol metabolism. Its utility spans basic research, clinical diagnostics, and drug development.

       
关键字: CYP27A1抗体;CYP27A1;CYP27A1 Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力, 拥有完整、科学、质量管理体系。并不断致力于技术创新,产品创新和管理创新,赢得了国内外广大用户的信任。
成立日期 2024-06-24 (2年) 注册资本 50万(元)
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 中间体,化学试剂 经营模式 贸易,工厂,试剂,定制,服务
  • 湖北瑞和宁生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:50万(元)
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:中间体。试剂
  • 公司地址:江汉区
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