同用名/别名（Synonym）
Interleukin-3; IL-3; Hematopoietic Growth Factor; Mast Cell Growth Factor; MCGF; Multipotential Colony-Stimulating Factor; P-Cell-Stimulating Factor; IL3
描述（Description）
IL-3 protein is mainly secreted by T lymphocytes, mast cells and osteoblasts and is critical for the generation and differentiation of hematopoietic progenitor cells. It stimulates mature basophils, eosinophils and monocytes, promoting functional activation. IL-3 Protein, Human (His-Avi) is the recombinant human-derived IL-3 protein, expressed by E. coli , with N-His, N-Avi labeled tag. The total length of IL-3 Protein, Human (His-Avi) is 133 a.a., with molecular weight of ~16.9 kDa.
纯度（Purity）
≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
生物活性（Bioactivity）
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.02-0.1 ng/mL.
氨基酸序列（Target Protein Sequence）
APMTQTTPLKTSWVNCSNMIDEIITHLKQPPLPLLDFNNLNGEDQDILMENNLRRPNLEAFNRAVKSLQNASAIESILKNLLPCLPLATAAPTRHPIHIKDGDWNEFRRKLTFYLKTLENAQAQQTTLSLAIF
制剂（Trait）
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.
复溶（Reconstitution）
It is recommended to redissolve in sterile deionized water.
保存条件&效期（Storage & Stability）
Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
运输方式(Shipping)
Room temperature in continental US; may vary elsewhere.
生物活性（Bioactivity） | 
Determined by its ability to inhibit the proliferation of mouse MPC-11 cells.The expected ED50 is≤ 2.0 ng/ml, corresponding to a specific bioactivity of≥5 x 105 units/mg.
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电泳（SDS-PAGE） | 
Recombinant Human IL-3 SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y02101 SDS-PAGE. |
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关键字： GMP级;高纯度;批间一致;生物活性;科研专供;
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苏州丰度依托先进技术平台，打造成熟生产体系，严控基因工程设计、细胞培养、蛋白纯化等环节，优化工艺与管理，确保产品高纯度、高活性且批间一致。产品覆盖基础研究、生物医药研发等多领域，为科研及生产提供多样、适配产品，助力突破技术难题 。