ECD抗体,Rabbit Polyclonal ECD Antibody
  • ECD抗体,Rabbit Polyclonal ECD Antibody
  • ECD抗体,Rabbit Polyclonal ECD Antibody
  • ECD抗体,Rabbit Polyclonal ECD Antibody

ECD抗体

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 湖北
更新日期 2025-05-14
QQ交谈 微信洽谈

产品详情

中文名称:ECD抗体英文名称:Rabbit Polyclonal ECD Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 5155 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Rabbit
偶联物: 靶点: ECD
2025-05-14 ECD抗体 Rabbit Polyclonal ECD Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 5155 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/40-1/200 Human,Mouse,Rat
ICC技术咨询 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa1/5000-1/10000 Human,Mouse,Rat
   

产品详情

AliasesNDGOA; PARK5; PGP95; SPG79; PGP9.5; Uch-L1; HEL-117; PGP 9.5; HEL-S-53
Entrez GeneID7345
clone1B4H3
WB Predicted band size24.8kDa
Host/IsotypeMouse IgG2b
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Rat
ImmunogenPurified recombinant fragment of human UCHL1 (AA: 1-220) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide

+ +

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       Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    


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       Western blot analysis using UCHL1 mouse mAb against mouse brain (1), and rat brain (2) cell lysate.    


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       Immunofluorescence analysis of Hela cells using UCHL1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    


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       Flow cytometric analysis of Hek293 cells using UCHL1 mouse mAb (green) and negative control (red).    


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       Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissues using UCHL1 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human brain tissues using UCHL1 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues using UCHL1 mouse mAb with DAB staining.    


           

参考文献

以下是3篇与ECD(细胞外结构域)抗体相关的研究文献概览:

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1. **文献名称**:*Anti-receptor antibodies in cancer therapy: Targeting EGFR and HER2*

**作者**:Yarden, Y., & Mendelsohn, J.

**摘要**:综述了针对EGFR和HER2细胞外结构域(ECD)的单克隆抗体(如西妥昔单抗、曲妥珠单抗)的作用机制,强调其通过阻断配体结合或诱导受体降解抑制肿瘤生长,并讨论其在乳腺癌和结直肠癌中的临床应用。

2. **文献名称**:*Trastuzumab: A model antibody-drug for HER2-positive breast cancer*

**作者**:Slamon, D. J., et al.

**摘要**:报道了靶向HER2蛋白ECD的单抗药物赫赛汀(曲妥珠单抗)的临床试验结果,证实其通过抑制HER2信号通路显著延长转移性乳腺癌患者生存期,奠定ECD抗体在靶向治疗中的地位。

3. **文献名称**:*PD-1/PD-L1 checkpoint blockade: Focus on the extracellular domain interactions*

**作者**:Topalian, S. L., & Keir, M. E.

**摘要**:分析PD-1/PD-L1免疫检查点抗体(如纳武利尤单抗)的作用,指出其通过结合ECD阻断免疫抑制信号,恢复T细胞活性,推动抗PD-1疗法在黑色素瘤等癌症中的突破。

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**备注**:上述文献为示例,实际引用时建议通过PubMed或Google Scholar核对具体作者及发表时间。

       

背景信息

ECD antibodies target the extracellular domain (ECD) of transmembrane proteins, which are critical components in cell signaling, adhesion, and receptor-ligand interactions. These antibodies are widely used in biomedical research and therapeutic development due to their ability to bind surface-exposed regions of proteins like receptor tyrosine kinases, cytokine receptors, or cell adhesion molecules. By blocking or modulating ECD-mediated functions, they help study protein activity, cellular communication, and disease mechanisms.

In therapeutics, ECD-specific antibodies are foundational for monoclonal antibody (mAb) drugs, such as trastuzumab (targeting HER2/neu in breast cancer) or cetuximab (anti-EGFR for colorectal cancer). They inhibit ligand binding, receptor dimerization, or downstream signaling, offering precision in cancer and autoimmune disease treatments. Diagnostic applications include biomarker detection in ELISA, flow cytometry, or immunohistochemistry, aiding disease diagnosis and monitoring.

ECD antibodies are typically generated using recombinant ECD fragments as immunogens. Their specificity depends on conformational epitopes, requiring careful validation to avoid cross-reactivity. Challenges include preserving native protein folding during antigen production and ensuring functional affinity. Advances in structural biology and phage display libraries have improved ECD antibody development, enhancing their utility in both research and clinical settings.

       
关键字: ECD抗体;ECD;ECD Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力, 拥有完整、科学、质量管理体系。并不断致力于技术创新,产品创新和管理创新,赢得了国内外广大用户的信任。
成立日期 2024-06-24 (2年) 注册资本 50万(元)
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 中间体,化学试剂 经营模式 贸易,工厂,试剂,定制,服务
  • 湖北瑞和宁生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:50万(元)
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:中间体。试剂
  • 公司地址:江汉区
询盘

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