TAF15抗体

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: A549 cells, Primary antibody: P01478(IMPDH2 Antibody) at dilution 1/650, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human lung cancer tissue using P01478(IMPDH2 Antibody) at dilution 1/60. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P01478(IMPDH2 Antibody) at dilution 1/60. (Original magnification: ×200)    

  
  

TAF15 (TATA-box binding protein-associated factor 15) is a member of the TET family of RNA-binding proteins, which also includes FUS and EWSR1. It functions as a component of the RNA polymerase II transcriptional machinery, playing roles in transcription initiation, RNA splicing, and DNA damage response. Structurally, TAF15 contains conserved RNA recognition motifs (RRMs) and a prion-like domain, enabling its participation in liquid-liquid phase separation and formation of membraneless organelles like stress granules.

Antibodies targeting TAF15 are critical tools for investigating its expression, localization, and molecular interactions. These antibodies are widely used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to study TAF15's involvement in cellular processes and disease pathogenesis. Dysregulation of TAF15 is linked to cancer and neurodegenerative disorders. For instance, chromosomal translocations involving TAF15 (e.g., *TAF15-NR4A3* in extraskeletal myxoid chondrosarcoma) generate oncogenic fusion proteins, while cytoplasmic mislocalization of TAF15 is observed in amyotrophic lateral sclerosis (ALS).

TAF15 antibodies are often validated for specificity against its unique N-terminal domain or shared C-terminal regions. Researchers also utilize these antibodies to explore post-translational modifications (e.g., phosphorylation) and interactions with partners like RNA polymerase II or spliceosome components. Their application advances understanding of TAF15's dual roles in transcription-coupled genomic stability and RNA metabolism.