"Daudi人Burkitt's淋巴瘤细胞全年复苏|已有STR图谱
传代比例:1:2-1:4(首次传代建议1:2)
生长特性:悬浮生长
当T25瓶复苏细胞收到货时,请观察好细胞状态后,将T25细胞瓶壁进行75%酒精消毒,将T25瓶置于37度培养箱放置2-4h,以便稳定细胞状态,当细胞密度达80%-90%,即可进行首次传代培养;干冰运输的细胞冻存管收到货后,需立即转入液氮保存或直接进行复苏(第三天换液并检查复苏细胞密度,以便进行下一步)。 能够在实验室条件下进行大量培养和繁殖。这种细胞系在分子生物学和生物技术研究中十分常用。
换液周期:每周2-3次
Leukemia L1210 Cells;背景说明:该细胞源于用0.2%甲基胆蒽(溶解)涂抹雌性小鼠的皮肤诱发的肿瘤,鼠痘病毒阴性。;传代方法:1:2传代;生长特性:悬浮生长;形态特性:淋巴母细胞样;相关产品有:Normal Rat, August 3, 1983细胞、NCI.H226细胞、HCGC细胞
V 79-4 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:FHs 74 Int细胞、K 562细胞、AU-565细胞
HuT-78 Cells;背景说明:皮肤;T淋巴瘤;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:悬浮;形态特性:详见产品说明书;相关产品有:LLC-PK1细胞、University of Arizona Cell Culture-812细胞、LS-513细胞
背景信息:1967年,该细胞系KleinE和KleinG建系,源于一名16岁患有Burkitt's淋巴瘤的黑人男性,beta-2-微球蛋白阴性,表达EBNA,VCA,sIg。该细胞携带EB病毒,是一个典型的B淋巴母细胞系,可用于白血病发病机制的研究。
Daudi人Burkitt's淋巴瘤细胞全年复苏|已有STR图谱
产品包装:复苏发货:T25培养瓶(一瓶)或冻存发货:1ml冻存管(两支)
贴壁细胞的传代培养,详细步骤如下:首先倒掉培养基,在这一步骤可以收集一些细胞上清做支原体检测;加入胰蛋白酶,一般T25是加2mL,盖好瓶盖,摇晃T25培养瓶,使胰蛋白酶均匀覆盖在细胞表面,放入培养箱2-3min,期间可在显微镜下观察,看到大部分细胞变圆,即可放入超净台,加入2倍的完全培养基,这里就是加4mL培养基,终止消化;将含有胰蛋白酶,细胞和培养基一起转移到离心管中,1000rpm/3min离心,去掉上清;新鲜的完全培养基重悬,根据细胞的生长特性和后续的实验需求进行传代,比如我养的Hepa1-6就长的比较快,不是着急用的话,我就会按1E6个细胞/T75培养瓶进行传代;但如果后两天要用,就会适当多传一点;还可通过显微镜计数后,直接用于细胞铺板,继续后续的实验。
LNCaP.FGC Cells;背景说明:人前列腺癌细胞LNCaP克隆FGC是从一位50岁白人男性(血型B+)的左锁骨淋巴结针刺活检中分离,该患者经确诊为前列腺癌转移。 这株细胞对5-α-二睾酮(生长调节子和酸性脂酶产物)有响应。这株细胞并不形成一致的单层,而是形成集落,在传代时可以用滴管反复吹吸打碎。它们仅仅轻轻地吸附在基底上,不形成汇合,很快使培养基变酸。生长很慢。传代后48小时内不应扰动。当培养瓶封包后,多数细胞从培养瓶底分离,悬浮在培养基中。收到后,在通常培养单层细胞的条件下培养24到48小时,以合细胞再贴壁。;传代方法:消化3-5分钟。1:2。3天内可长满。;生长特性:贴壁生长;形态特性:上皮细胞;相关产品有:NB-19细胞、B10BR细胞、SW-1417细胞
RFL 6 Cells;背景说明:胚肺;成纤维细胞;SD大鼠;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:BT 325细胞、HEC1-A细胞、SUM52细胞
HuH7 Cells;背景说明:详见相关文献介绍;传代方法:消化3-5分钟。1:2。3天内可长满。;生长特性:贴壁生长;形态特性:上皮样;相关产品有:CAL-120细胞、MeT 5A细胞、M 1细胞
A-1-19 Cells(提供STR鉴定图谱)
来源说明:细胞主要来源ATCC、ECACC、DSMZ、RIKEN等细胞库
物种来源:人源、鼠源等其它物种来源
Daudi人Burkitt's淋巴瘤细胞全年复苏|已有STR图谱
形态特性:淋巴母细胞样
细胞复苏相关注意事项:1.取细胞的过程中注意带HAO防冻手套,护目镜。此项尤为重要,细胞冻存管可能漏入,解冻时冻存管中的气温急剧上升,可导致爆炸。2.冻存的问题:冻存的配置已是常识,在这里不作详述,但二甲基亚砜(DMSO )对细胞不是完全无毒副作用,在常温下,二甲基亚砜对细胞的毒副作较大,因此,必须在1-2min内使冻存完全融化。如果复苏温度太慢,会造成细胞的损伤,二甲基亚砜(DMSO)ZuiHAO选择进口产品。3.离心前须加入少量培养。细胞解冻后二甲基亚砜浓度较GAO,注意加入少量培养可稀释其浓度,以减少对细胞的损伤。4.离心问题:目前主要有两种见解。一种是解冻后的细胞悬直接吹打均匀后分装到培养瓶中进行培养,第二天换。因为离心的目的是两个,去除DMSO,去除死细胞,这个是标准流程,但对一般人来说,把握不HAO离心转速和时间,转的不够活细胞沉底的少,细胞就全被扔掉了,转过了活细胞会受压过大,死亡。此外在操作过程中容易污染,所以不推荐。另一种说法为细胞悬中含有二甲基亚砜(DMSO),DMSO对细胞有一定的毒副作用,所以须将离心后的体前倒净,且一定倒干净。我在试验中按照常规的离心分装的方法进行复苏,结果无异常。5.细胞贴壁少的问题:教科书中说明冻存细胞解冻时1ml细胞要加10ml-15ml培养,而在我的试验中的经验总结为培养基越少细胞越容易贴附。6.复苏细胞分装的问题:试验中我的经验总结为复苏1管细胞一般可分装到1-2只培养瓶中,分装过多,细胞浓度过低,不利于细胞的贴壁。7.加培养基的量放入问题:这个量的多少的把握主要涉及到的问题DMSO的浓度,从如果你加培养基的太少,那么DMSO的浓度就会比较大,就会影响细胞生长,从以前的资料来看,DMSO的浓度在小于0.5%的时候对一般细胞没有什么影响,还有一个说法是1%。所以如果你的冻存的浓度是10%DMSO的话那么加10ml以上的培养基就恰HAO稀释到了无害浓度。
CESS Cells;背景说明:急性髓系白血病;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:悬浮;形态特性:详见产品说明书;相关产品有:EFM-19细胞、B16 melanoma细胞、CaSki细胞
SNU-719 Cells;背景说明:胃癌;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:HcerEpic细胞、P36细胞、Sp2/0-Ag14细胞
alpha-TC1.6 Cells;背景说明:胰岛素瘤;a细胞;C57BL/6xDBA/2;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:Line 522细胞、MV 4;11细胞、Leydig细胞
NALM6 Cells;背景说明:急性B淋巴细胞白血病;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:悬浮;形态特性:详见产品说明书;相关产品有:Ramos (RA)细胞、IPEC-J2细胞、CTV1细胞
Bac12F5 Cells;背景说明:巨噬细胞;SV40转化;BALB/c x A.CA;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:RERF-LCMS细胞、WEHI 164细胞、H-28细胞
RS 4;11 Cells;背景说明:详见相关文献介绍;传代方法:每周2-3次;生长特性:悬浮生长;形态特性:成淋巴细胞;相关产品有:MPC5细胞、H-1404细胞、D-341 Med细胞
ACC-3 Cells;背景说明:Acc-3细胞源自一位49岁男性的腺样囊性癌。能表达角蛋白。;传代方法:消化3-5分钟。1:2。3天内可长满。;生长特性:贴壁生长;形态特性:上皮样;相关产品有:4T1-LUC细胞、HRA 19细胞、SUM-149细胞
3T3-F442A Cells;背景说明:脂肪前体细胞;雄性;Swiss albino;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:rRTEC细胞、SK-LU-1细胞、Panc 05.04细胞
PANC 203 Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁生长;形态特性:上皮样;相关产品有:TE4细胞、WEHI-3细胞、L-WRN细胞
BALL-1 Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:悬浮生长;形态特性:淋巴母细胞;相关产品有:HepG2细胞、SACC-LM细胞、LCLC-103H细胞
NCIH1770 Cells;背景说明:详见相关文献介绍;传代方法:随细胞的生长而换液;生长特性:悬浮生长;形态特性:详见产品说明书;相关产品有:J45.01细胞、293 F细胞、Kasumi 1细胞
GM03671 Cells;背景说明:G.E. Foley 等人建立了类淋巴母细胞细胞株CCRF-CEM。 细胞是1964年11月从一位四岁白人女性急性淋巴细胞白血病患者的外周血白血球衣中得到。此细胞系从香港收集而来。;传代方法:1:2传代。3天内可长满。;生长特性:悬浮生长;形态特性:淋巴母细胞样;相关产品有:TE-3A细胞、NCI-H295R细胞、E304细胞
118MG Cells;背景说明:注意: 据报道来自不同个体的胶质母细胞瘤细胞株U-118 MG (HTB-15) 和 U-138 MG (HTB-16)有着一致的VNTR和相近的STR模式。 U-118 MG 和 U-138 MG细胞遗传学上很相似并有至少六个衍生标记染色体。 这是1966年至1969年间J. Ponten和同事从恶性神经胶质瘤中构建的细胞株中的一株(其它包括ATCC HTB-14和 ATCC HTB-16 and ATCC HTB-17)。 1987年用BM-Cycline培养6周去除了支原体污染。 ;传代方法: 消化3-5分钟。1:2传代。3天内可长满。;生长特性:贴壁生长;形态特性:混合型;相关产品有:SUP-B1细胞、Detroit 562细胞、LM1细胞
KU-19-19 Cells;背景说明:膀胱癌;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:MM1.S细胞、SK-NEP-1细胞、HT细胞
SKMEL1 Cells;背景说明:详见相关文献介绍;传代方法:维持细胞浓度在1×105-2×105,每周补液2-3次。;生长特性:悬浮生长;形态特性:球形的;相关产品有:HL-60 Clone 15细胞、HCC1954BL细胞、CHO-Lec1细胞
COC1/CDDP Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:UCLA RO-81A-1细胞、IFRS1细胞、DHL-6细胞
H-2405 Cells;背景说明:详见相关文献介绍;传代方法:1:5-1:8传代;生长特性:混合生长;形态特性:详见产品说明书;相关产品有:Lu-99A细胞、WiDr细胞、WM115F细胞
45C6A Cells(提供STR鉴定图谱)
Abcam Jurkat SH2D1A KO Cells(提供STR鉴定图谱)
AMC-106 Cells(提供STR鉴定图谱)
BayGenomics ES cell line RRK166 Cells(提供STR鉴定图谱)
BayGenomics ES cell line XL528 Cells(提供STR鉴定图谱)
CaHo-26 Cells(提供STR鉴定图谱)
DA00533 Cells(提供STR鉴定图谱)
EDi001-B-2 Cells(提供STR鉴定图谱)
GM05340 Cells(提供STR鉴定图谱)
MDA-MB231 Cells;背景说明:MDA-MB-231来自患有转移乳腺腺癌的51岁女病人的胸水。在裸鼠和ALS处理的BALB/c小鼠中,它能形成低分化腺癌(III级)。;传代方法:消化3-5分钟,1:2,3天内可长满;生长特性:贴壁生长;形态特性:上皮样;相关产品有:SF-126细胞、NCI-H1184细胞、TW-039细胞
CaSki Cells;背景说明:这株细胞是从小肠肠系膜转移灶的细胞中建立的。 据报道,它含有完整的HPV-16(每个细胞大约600个拷贝)和HPV-18相关序列。;传代方法:1:2传代;生长特性:贴壁生长;形态特性:上皮细胞样;相关产品有:HSF2细胞、EL 4细胞、KMS26细胞
NCI-SNU-878 Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:VERO C 1008细胞、CCRF-CEM C7细胞、COR L279细胞
Metastatic Variant 522 Cells;背景说明:肺腺癌;女性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:ZR-75-30细胞、MV3细胞、MKN-74细胞
SW48 Cells;背景说明:1971-1975年间,A. Leibovitz从结肠腺癌中分离建立十个细胞系,SW48为为其中之一。该细胞合成癌胚抗原,能在裸鼠中成瘤。;传代方法:1:3传代,2-3天传一代;生长特性:贴壁生长;形态特性:上皮细胞样;相关产品有:NCI-H2452细胞、LAN5细胞、RS4:11细胞
COV-434 Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁生长;形态特性:上皮细胞;相关产品有:HT-1376细胞、PNT-1A细胞、B16BL6细胞
Swiss/3T3 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:Human Fetal Thymocyte-8810细胞、LL/2 (LLC1)细胞、NCI-H2085细胞
Human Melanoma Cell Bowes Cells;背景说明:详见相关文献介绍;传代方法:1:6—1:10传代,2天换液1次;生长特性:贴壁生长;形态特性:上皮细胞;相关产品有:Jeko1细胞、hEM15A细胞、Ca 9-22细胞
Daudi人Burkitt's淋巴瘤细胞全年复苏|已有STR图谱
Colo699 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:H-2330细胞、H-661细胞、HCT116/L细胞
hTERT-HME1 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:A-673细胞、COLO 320 HSR细胞、NL-20细胞
M14-MEL Cells;背景说明:详见相关文献介绍;传代方法:1:3传代;生长特性:混合生长;形态特性:详见产品说明书;相关产品有:MDA-MB 361细胞、SW982细胞、CCD-966SK细胞
Hs 934.T Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代,;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:成纤维细胞;相关产品有:MV4-11细胞、NCIH2030细胞、G 401细胞
COV434 Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁生长;形态特性:上皮细胞;相关产品有:SRS-82细胞、NWA细胞、FL83B细胞
H596 Cells;背景说明:详见相关文献介绍;传代方法:1:4-1:8传代;每周换液2-3次。;生长特性:贴壁生长;形态特性:上皮样;相关产品有:GA-10(Clone 4)细胞、CCD 19Lu细胞、Dakiki细胞
MCF-12A Cells;背景说明:非致瘤性乳腺上皮细胞;女性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:LSC-1细胞、SKHEP-1细胞、EB-2细胞
HAP1 AGL (-) 2 Cells(提供STR鉴定图谱)
HAP1 SIK1 (-) 2 Cells(提供STR鉴定图谱)
OVCA420 Cells;背景说明:卵巢癌;女性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:H 9细胞、MGH-U1细胞、CEM-CCRF细胞
KRC/Y Cells;背景说明:肾透明细胞癌;女性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:VMM5细胞、GT1-1细胞、P31/FUJ细胞
SKml2 Cells;背景说明:详见相关文献介绍;传代方法:1:3-1:6传代,2-3天换液1次。;生长特性:贴壁生长;形态特性:多边形的;相关产品有:P3/X63-Ag8细胞、C-32细胞、MOLM16细胞
CC-LP-1 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:HCC-2279细胞、TE-11细胞、HEC1B细胞
HEK-293F Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁生长;悬浮生长;形态特性:上皮细胞样;相关产品有:Jurkat E6细胞、FTC-133细胞、COLO 320DM细胞
NCI-H2330 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:SW 403细胞、NCI-H820细胞、MFC细胞
KP 4 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁生长;形态特性:详见产品说明书;相关产品有:Saos2细胞、D341Med细胞、CCD 19Lu细胞
Huh-7 Cells;背景说明:详见相关文献介绍;传代方法:消化3-5分钟。1:2。3天内可长满。;生长特性:贴壁生长;形态特性:上皮样;相关产品有:MCM细胞、EAhy926细胞、MDAMB435S细胞
HyCyte MDA-MB-231 KO-hHACD2 Cells(提供STR鉴定图谱)
LCL-PI 14 Cells(提供STR鉴定图谱)
NA Cells(提供STR鉴定图谱)
OVXF 1544 Cells(提供STR鉴定图谱)
RTE2 Cells(提供STR鉴定图谱)
TR138 Cells(提供STR鉴定图谱)
UKBi013-A Cells(提供STR鉴定图谱)
HG01063 Cells(提供STR鉴定图谱)
CD 18 Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁生长;形态特性:详见产品说明书;相关产品有:KU1919细胞、Earle's L cells细胞、HR-8348细胞
SK-ML2 Cells;背景说明:详见相关文献介绍;传代方法:1:3-1:6传代,2-3天换液1次。;生长特性:贴壁生长;形态特性:多边形的;相关产品有:COR L279细胞、Sp2/0-Ag14细胞、J 111细胞
Mhh-Call 2 Cells;背景说明:急性B淋巴细胞白血病;女性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:悬浮;形态特性:详见产品说明书;相关产品有:GP2-293细胞、RBVEC细胞、J 774A.1细胞
S180 Cells;背景说明:小鼠肉瘤;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:半贴壁;形态特性:详见产品说明书;相关产品有:SK-N-BE(2)细胞、HT clone H9细胞、NCI-H1975细胞
NCI H157 Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁生长 ;形态特性:详见产品说明书;相关产品有:Hs-606-T细胞、HPAF/CD18细胞、KGN细胞
NCI H157 Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁生长 ;形态特性:详见产品说明书;相关产品有:Hs-606-T细胞、HPAF/CD18细胞、KGN细胞
COLO 320 HSR Cells;背景说明:该细胞1984年建系,源自一位33岁患有大肠腺癌男性经5-fu治疗后的腹水。;传代方法:1:2传代。3天内可长满。;生长特性:半贴壁生长;形态特性:详见产品说明书;相关产品有:DR2R1610细胞、E0771细胞、H-498细胞
293GP Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:BT.549细胞、H-23细胞、R3/1细胞
T2 (174 x CEM.T2) Cells;背景说明:详见相关文献介绍;传代方法:1:3—1:6传代,每周换液2—3次;生长特性:悬浮生长;形态特性:淋巴母细胞样;相关产品有:Bowes melanoma cells细胞、NCIH2347细胞、GP2d细胞
AsPC-1 Cells;背景说明:该细胞来源于人胰腺癌裸鼠异种移植产生的癌性腹水,可以表达CEA,人胰腺相关抗原、人胰腺特异性抗原和黏蛋白。;传代方法:1:3-1:6传代;每周换液2-3次。;生长特性:贴壁生长;形态特性:上皮样;相关产品有:MCF12A细胞、HCT-8/V细胞、LSECs细胞
NCI.H226 Cells;背景说明:1980年分离建立。;传代方法:1:2传代;生长特性:贴壁生长;形态特性:上皮细胞样;相关产品有:HEC1-B细胞、5-8F细胞、NCI H508细胞
NCI-841 Cells;背景说明:详见相关文献介绍;传代方法:1:3—1:5传代,;生长特性:混合型生长;形态特性:详见产品说明书;相关产品有:AML-2细胞、Rat1细胞、LS180细胞
SVEC4-10 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:Hx147细胞、CORL88细胞、Hs 729.T细胞
CCD-966SK Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:P3X63Ag8653细胞、H-1836细胞、PJ34细胞
HCC1954 BL Cells;背景说明:外周血B淋巴细胞;EBV转化;女性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:悬浮;形态特性:详见产品说明书;相关产品有:Sun Yat-sen university Ophtalmic center-Retinoblastoma 50细胞、HPC-Y5细胞、SW 900细胞
TER347 Cells(提供STR鉴定图谱)
HEK 293-H Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:Hs870T细胞、KYSE510细胞、PLA801-95C细胞
NCM-460 Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁生长;形态特性:详见产品说明书;相关产品有:H-2227细胞、H-220细胞、Roswell Park Memorial Institute 8226细胞
HMC-1 Cells;背景说明:肥大 Cells;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:悬浮;形态特性:详见产品说明书;相关产品有:ML-2细胞、NCI-H711细胞、H35 Reuber细胞
GM06141B Cells;背景说明:这株淋巴母细胞样细胞株,源自一位30岁白人男性一,患有恶性红细胞白血病,能够自然产生并能诱导球蛋白合成。细胞的EB病毒核抗原阴性,没有表面免疫球蛋白与细胞质免疫球蛋白。HEL细胞表达HLA抗原(HLA-A3,AW32,BW35),β-2小球蛋白,一定比例的细胞还表达Ia抗原。这个细胞株提供了一种用于研究红细胞分化和球蛋白基因表达的模型。它类似于小鼠中的血友病。;传代方法:1:2传代。3天内可长满。;生长特性:悬浮生长;形态特性:淋巴母细胞样;相关产品有:MGH-U1 (EJ)细胞、SN12-PM6细胞、H-719细胞
32D/cl3 Cells;背景说明:骨髓淋巴瘤;C3H/HeJ;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:悬浮;形态特性:详见产品说明书;相关产品有:H4-II-E-C3细胞、Hs746-T细胞、EM-3细胞
LAPC4 Cells;背景说明:前列腺癌;淋巴结转移;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:EVSAT细胞、SNU886细胞、Bovine ENDometrial cells细胞
Hs-611-T Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;每周换液2-3次。;生长特性:混合型;形态特性:淋巴母细胞样;相关产品有:HCC-202细胞、H-1618细胞、Sol8细胞
H920 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:HG2855细胞、SPCA-1细胞、MA-c细胞
Daudi人Burkitt's淋巴瘤细胞全年复苏|已有STR图谱
CCD841CoN Cells;背景说明:结肠上皮细胞;女性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:Molm 14细胞、Hep2细胞、Hs742T细胞
Colon26 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:ABE8.1/2细胞、H4IIEC3细胞、WiDr-TC细胞
BNCL2 Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁生长 ;形态特性:详见产品说明书;相关产品有:H647细胞、H1793细胞、HCMEC(BL12-H)细胞
CLL-CII Cells;背景说明:慢性淋巴细胞白血病;女性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:悬浮;形态特性:详见产品说明书;相关产品有:SW13细胞、MSC细胞、ChaGo-K-1细胞
VAESBJ Cells;背景说明:详见相关文献介绍;传代方法:1:3传代,每周2次;生长特性:贴壁生长;形态特性:上皮细胞样;相关产品有:NCIADRRES细胞、639V细胞、D324细胞
KOSC-2 cl3-43 Cells;背景说明:详见相关文献介绍;传代方法:2.2 x 10^4 cells/ml ;生长特性:贴壁生长;形态特性:上皮样;相关产品有:RS 4;11细胞、HOC1细胞、MFE 296细胞
BayGenomics ES cell line CSD073 Cells(提供STR鉴定图谱)
BayGenomics ES cell line RRS748 Cells(提供STR鉴定图谱)
BayGenomics ES cell line YTC145 Cells(提供STR鉴定图谱)
iMOSEC-2 Cells(提供STR鉴定图谱)
PCRP-SMAD4-1H2 Cells(提供STR鉴定图谱)
LO-CD4-a Cells(提供STR鉴定图谱)
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Maurer B.A., Imamura T., Wilbert S.M.
Incidence of EB virus-containing cells in primary and secondary clones of several Burkitt lymphoma cell lines.
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Klein G., Dombos L., Gothoskar B.
Sensitivity of Epstein-Barr virus (EBV) producer and non-producer human lymphoblastoid cell lines to superinfection with EB-virus.
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Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis.
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Differential expression of the amv gene in human hematopoietic cells.
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The absence of beta 2-microglobulin in Daudi cells: active gene but inactive messenger RNA.
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The beta2-microglobulin mRNA in human Daudi cells has a mutated initiation codon but is still inducible by interferon.
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Brodsky F.M.
A matrix approach to human class II histocompatibility antigens: reactions of four monoclonal antibodies with the products of nine haplotypes.
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Ehlin-Henriksson B., Klein G.
Distinction between Burkitt lymphoma subgroups by monoclonal antibodies: relationships between antigen expression and type of chromosomal translocation.
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Relationship between karyotype of tissue culture lines and tumorigenicity in nude mice.
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Cell surface antigens of human ovarian and endometrial carcinoma defined by mouse monoclonal antibodies.
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Distinct reactivity of Burkitt's lymphoma cell lines with eight monoclonal antibodies correlated with the ethnic origin.
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The t(8;14) chromosome translocation of the Burkitt lymphoma cell line Daudi occurred during immunoglobulin gene rearrangement and involved the heavy chain diversity region.
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关键字: Daudi人Burkitt's淋巴瘤细胞;传代细胞;复苏细胞;实验细胞;科研细胞;
上海冠导生物工程有限公司,先后从ATCC、DSMZ、ECACC、RIKEN、PromoCell、ScienCell、JCRB等国内外细胞库引进细胞2000余株。以此为契机,公司组建了冠导细胞库,我司细胞均由资深细胞培养工程师进行培养。我司可以提供的细胞有:①细胞系②原代细胞③稳转株④耐药株⑤标记细胞⑥细胞配套试剂等。