114162-64-0 X-Glc 5-溴-4-氯-3-吲哚基-β-D-葡萄糖苷酸环己铵盐

95%的大肠杆菌具有β-葡萄糖苷酶基因，用这种发色底物的培养基可以检测以及定量食品样本如肉、奶制品以及贝类中的大肠杆菌数量，以及在临床上检测尿路感染。国际上普遍采用该产品取代传统方法以精确检测饮用水中的大肠杆菌数量，该方法大大降低了假阳性和假阴性。该产品也用于快速检测植物中GUS基因融合标记。β-葡糖醛酸酶（β-glucuronidase (GUS)）基因检测的显色底物。

X-GlcA is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. X-GlcA is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype. When X-GlcA is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of X-GlcA turn an intense blue color, allowing for easy differentiation between lac+ and lac- colonies.
Chromogenic substrate for β-glucuronidase (GUS) gene detection. Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype.