The WDR77 antibody is a crucial tool for studying the WD repeat-containing protein 77 (WDR77), also known as MEP50 or androgen receptor cofactor p44. WDR77 is a conserved scaffolding protein characterized by WD40 repeats, which mediate protein-protein interactions. It plays diverse roles in cellular processes, including transcriptional regulation, RNA processing, and chromatin remodeling. Notably, WDR77 forms a complex with protein arginine methyltransferase 5 (PRMT5), functioning as a methyltransferase module that catalyzes symmetric dimethylation of histones and other proteins, influencing gene expression and cell cycle progression.
Research highlights its involvement in development and disease. WDR77 is essential for embryonic development and spermatogenesis, while dysregulation is linked to cancers (e.g., prostate, lung, and breast cancers) due to its role in promoting cell proliferation and survival. The antibody enables detection of WDR77 expression, localization, and interaction partners in techniques like Western blotting, immunohistochemistry, and co-immunoprecipitation. Validated antibodies help investigate its oncogenic mechanisms, such as modulating androgen receptor signaling in prostate cancer. Host species (e.g., rabbit, mouse) and epitope specificity vary across commercial antibodies, requiring validation via knockout controls. Its study contributes to understanding epigenetic regulation and potential therapeutic targeting in malignancies.