GPR15抗体;GPR15 Antibody—艾普蒂

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     Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    

  
  

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     Immunofluorescence analysis of Hela cells using GPR15 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

  
  

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     Flow cytometric analysis of Raji cells using GPR15 mouse mAb (green) and negative control (red).    

  
  

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     Flow cytometric analysis of Jurkat cells using GPR15 mouse mAb (green) and negative control (red).    

  
  

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     Flow cytometric analysis of THP-1 cells using GPR15 mouse mAb (green) and negative control (red).    

  
  

GPR15 (G protein-coupled receptor 15) is an orphan receptor belonging to the class A GPCR family, initially identified for its role in T-cell homing to the colon and skin. It gained attention due to its involvement in immune regulation and inflammation, particularly in mucosal tissues. GPR15 functions as a co-receptor for simian immunodeficiency virus (SIV) and certain HIV strains, though its role in human HIV infection remains less defined. In immunology, GPR15⁺ regulatory T cells (Tregs) are implicated in maintaining gut homeostasis and suppressing inappropriate immune responses. Dysregulation of GPR15 signaling has been associated with inflammatory bowel disease (IBD), psoriasis, and other autoimmune conditions.

Antibodies targeting GPR15 are crucial research tools for investigating receptor expression, localization, and function. They enable detection of GPR15 on immune cells via flow cytometry, immunohistochemistry, and Western blotting. Some studies explore therapeutic potential, as modulating GPR15 activity could influence T-cell migration or viral entry. However, challenges persist due to GPR15's orphan status (unknown endogenous ligand) and species-specific differences in function. Current antibody validation emphasizes specificity checks in knockout models to avoid cross-reactivity with related GPCRs. Research continues to clarify its physiological ligands and signaling pathways, positioning GPR15 antibodies as essential reagents for decoding its roles in immunity and disease.