Human FUS Protein, MBP,His Tag
分子别名（Synonym）
75 kDa DNA-pairing protein,ALS6,amyotrophic lateral sclerosis 6,FUS1,fused in sarcoma,fus-like protein,Oncogene FUS,Oncogene TLS,RNA-binding protein FUS,POMP75,Translocated in liposarcoma,Translocated in liposarcoma protein
表达区间及表达系统（Source）
Human FUS Protein, MBP,His Tag (FUS-H5144) is expressed from E. coli cells. It contains AA Met 1 - Tyr 526 (Accession # P35637-1).
Predicted N-terminus: Met
蛋白结构（Molecular Characterization）
This protein carries a MBP tag at the N-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 97.0 kDa. The protein migrates as 95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>85% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景介绍
FUS (RNA-binding protein FUS) is a DNA/RNA-binding protein that plays a role in various cellular processes such as transcription regulation, RNA splicing, RNA transport, DNA repair and damage response. It binds to nascent pre-mRNAs and acts as a molecular mediator between RNA polymerase II and U1 small nuclear ribonucleoprotein thereby coupling transcription and splicing. It binds also its own pre-mRNA and autoregulates its expression; this autoregulation mechanism is mediated by non-sense-mediated decay.
FUS plays a role in DNA repair mechanisms by promoting D-loop formation and homologous recombination during DNA double-strand break repair. In neuronal cells, FUS plays crucial roles in dendritic spine formation and stability, RNA transport, mRNA stability and synaptic homeostasis.
关键字： FUS;FUS蛋白;FUS重组蛋白;ACRO;百普赛斯;
百普赛斯集团ACROBiosystems Group（股票代码：301080）是成立于2010年的跨国生物科技公司，是为全球生物医药、健康产业领域提供关键生物试剂产品及解决方案的行业平台型基石企业。2021年在创业板上市。百普赛斯集团业务遍布全球，横跨亚洲、北美洲、欧洲，在中国、美国、瑞士等12个城市设有办公室、研发中心及生产基地。目前累计服务客户超6000家，与全球Top 20医药企业均建立了长期、稳定的合作伙伴关系。集团旗下拥有品牌ACROBiosystems百普赛斯、bioSeedin柏思荟、Condense Capital垦拓资本和ACRODiagnostics百斯医学等。