单细胞测序用液滴微球产生的FluoSurf 2w/w in HFE7500表活氟油

基于液滴的微流体技术是一种新兴的强大工具，适用于dPCR或单细胞分析等生物应用。作为微反应器的液滴，可以在微流控芯片中产生，具有非常高的吞吐量，可在较短的时间内进行大量的平行实验。

氟化油通常用作这类应用的连续相，因为其能够溶解和输送氧气，并且不溶解大多数化学和生化化学物。

FluoSurf 是一种生物相容性氟化表面活性剂，专为生产单分散油包水液滴而设计和优化。FluoSurf 可确保液滴的长期稳定性（即使在极端条件下也是如此）。

优势

● 稳定性

   即使在dPCR或长期细胞培养实验等具有挑战性的条件下，也能实现单分散液滴群体和低液滴融合。

● 生物相容性

   经细胞检测验证的生物相容性，适用于生物实体。

● 批量生产

   我们的大批量生产能力使我们能够满足您的所有需求

● 较高纯度

   在制造加工过程中去除杂质

● 泄露控制：液滴内的分散相物质较少量的渗透到液滴外部

● 批次间重现性

   每个批次都遵循严格的质量控制分析，通过验证结构和微流体性能来确保一致性。

FluoSurf 在高温处理（例如PCR的加热循环）后仍保证液滴稳定性和群体单分散性。

应用领域

● 数字液滴PCR

   基于对液滴内大量分隔PCR反应的统计分析的超灵敏核酸定量方法

● 单细胞分析

   在受控环境下处理和分析单分散液滴群体中的分离细胞

● 筛选

   在微滴内以高通量对细胞、基因或生物大分子进行受控操作、分析和分选。

● 合成生物学

   使用微液滴作为细胞大小的隔间来模拟代谢反应。

● 控制化学和生物反应

   微流体工具专门设计用于增强对封装在液滴内的反应的控制。

规格参数

案例1: 液滴产生和热循环对尺寸分布的影响

实验条件：氟油流速为5μL/min，水相流速为1.66μL/min。

4w/w%表面活性剂HFE7500

热循环条件：30个循环

95℃驻留30s

55℃驻留1min

72℃驻留5min

40℃驻留1min

热循环前

热循环后

FluoSurf在热循环期间允许液滴具有良好的稳定性

使用FluoSurf表面活性剂，热循环对液滴尺寸分布的影响十分有限。

案例2：保留性能

荧光素100μM的全/空液滴平均强度的演变

FluoSurf限制液滴之间的交换（4天后少于10%的荧光素交换）

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