EGFR(L861Q)
[100 µL]
Catalog Number: CR-M26077
Gene Symbol: EGFR/HER1/ERBB1
Description: Anti EGFR(L861Q) Mouse Monoclonal Antibody
Background: EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR).
Immunogen: A synthetic peptide from the internal region of EGFR which includes the mutation of L861Q, human origin.
Tested Applications: ELISA, WB, IF, IHC
Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:1000-1:5000
IHC: 1:50-1:200
Concentration: 1 mg/ml
Host: Mouse
Format: Liquid
Clonality: Monoclonal
Isotype:
Purity: Purified from ascites
Storage buffer:
Preservative: no
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Recognizes EGFR(L861Q), but not wild type EGFR from vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles.
Western blot:
Western blot analysis of recombinant EGFR(L861Q) and wildtype proteins. Purified His-tagged EGFR(L861Q) protein (amino acids 702-987, lane 2, Cat. # CR-P10211) and corresponding wild type protein (lane 1, Cat. # CR-P10210) were blotted with anti-EGFR(L861Q) mouse monoclonal antibody (Cat. # CR-M26077). Proteins loading (bottom) were blotted with anti-His-tag monoclonal antibody (Cat. #28004).
Immunofluorescence:
Immunofluorescence of cells expressing EGFR proteins with anti EGFR(L861Q) antibody. HEK293T cells were transfected with pCDNA3-GFP EGFR(WT) plasmid (left column) or pCDNA3-GFP EGFR(L861Q) plasmid (right column), then fixed and stained with anti- EGFR(L861Q) monoclonal antibody (Cat. #CR-M26077).
关键字： 点突变抗体;抗体;
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