| 产品介绍 | For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indoly?l phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.碱性磷酸酶(alkaline phosphatase)的底物。 5-溴-4-氯-3-吲哚磷酸(BCIP) 和 氯化硝基四氮唑蓝(NBT)用于比色法鉴定碱性磷酸酶标记分子. BCIP/NBT 底物系统用途非常广泛：包括 Northern Southern, Western blotting, 原位杂交（in situ hybridization）和免疫组织化学。 BCIP 可以有两种盐的形式: 二钠盐型溶于水，对甲苯胺型溶于二甲基甲酰胺（DMF）。这些盐型可用于制备和NBT结合的储存溶液和反应缓冲液，构成碱性磷酸酶的底物溶液。 底物系统和碱性磷酸酶一起培养，产生一种不溶的带紫色的NBT双甲腊( diformazan )产物，容易被观察。 For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indoly?l phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
Histochemical substrate for alkaline phosphatase. |
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