描述（Description）
RSPO1 is a secreted protein,containing 2 FU(furin-like) repeats and 1 TSP type-1 domain and belonging to the R-spondin family. RSPO1 is required for the early development of gonads, regardless of sex. It has been found in mice only eleven days after fertilization. To induce cell proliferation, it acts synergistically with WNT4. They help stabilize β catenin, which activates downstream targets. RSPO1 is necessary in female sex development. It augments the WNT/β catenin pathway to oppose male sex development. In critical gonadal stages, between six and nine weeks after fertilization, the ovaries upregulate it while the testes downregulate it. RSPO1 can potentially aid in the treatment of mucositis, which is characterized by inflammation of the oral cavity. This unfortunate condition often accompanies chemotherapy and radiation in cancer patients with head and neck tumors.
纯度（Purity）
≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
生物活性（Bioactivity）
Measured by its ability to induce Topflash reporter bioactivity in CHO cells in the presence of 50ng/ml wnt3a. The expected ED50 is <1.0 μg/mL
氨基酸序列（Target Protein Sequence）
SRGIKGKRQRRISAEGSQACAKGCELCSEVNGCLKCSPKLFILLERNDIRQVGVCLPSCPPGYFDARNPDMNKCIKCKIEHCEACFSHNFCTKCKEGLYLHKGRCYPACPEGSSAANGTMECSSPAQCEMSEWSPWGPCSKKQQLCGFRRGSEERTRRVLHAPVGDHAACSDTKETRRCTVRRVPCPEGQKRRKGGQGRRENANRNLARKESKEAGAGSRRRKGQQQQQQQGTVGPLTSAGPA
制剂（Trait）
Lyophilized after extensive dialysis against PBS, pH 7.4.
复溶（Reconstitution）
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
保存条件&效期（Storage & Stability）
Stored at -20°C for 1 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.Please avoid repeated freeze-thaw cycles.
运输方式(Shipping)
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
生物活性（Bioactivity）
Measured by its ability to induce Topflash reporterBioactivity in CHO cells in the presence of 50ng/ml wnt3a. The expected ED50 is＜1.0 μg/ml. The bioactivity of Recombinant Human R-Spondin-1(cat noF1d0056) was higher than other competing products.
Highly consistent bioactivity between different production batches of Recombinant Human R-Spondin1 (Cat. No. F1d0056).
电泳（SDS-PAGE）
Recombinant Human R-Spondin1 (Cat. No. F1d0056) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y03501 SDS-PAGE.
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