描述（Description）
Fetuin A (or AHSG protein) plays multiple roles in cellular processes, promotes endocytosis and displays opsonic properties. It regulates the mineral phase of bone, affects bone homeostasis, and has significant affinity for calcium and barium ions. Fetuin A/AHSG Protein, Human (HEK293, His) is the recombinant human-derived Fetuin A/AHSG protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Fetuin A/AHSG Protein, Human (HEK293, His) is 349 a.a., with molecular weight of ~60.0 kDa.
生物活性（Bioactivity）
Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 20-40 ng/ml.
氨基酸序列（Target Protein Sequence）
APHGPGLIYRQPNCDDPETEEAALVAIDYINQNLPWGYKHTLNQIDEVKVWPQQPSGELFEIEIDTLETTCHVLDPTPVARCSVRQLKEHAVEGDCDFQLLKLDGKFSVVYAKCDSSPDSAEDVRKVCQDCPLLAPLNDTRVVHAAKAALAAFNAQNNGSNFQLEEISRAQLVPLPPSTYVEFTVSGTDCVAKEATEAAKCNLLAEKQYGFCKATLSEKLGGAEVAVTCTVFQTQPVTSQPQPEGANEAVPTPVVDPDAPPSPPLGAPGLPPAGSPPDSHVLLAAPPGHQLHRAHYDLRHTFMGVVSLGSPSGEVSHPRKTRTVVQPSVGAAAGPVVPPCPGRIRHFKV
制剂（Trait）
Lyophilized from a 0.22 μm-filtered solution containing 10 mM PB, pH 7.4.
复溶（Reconstitution）
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
保存条件&效期（Storage & Stability）
Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
运输方式(Shipping)
Room temperature in continental US; may vary elsewhere.
生物活性（Bioactivity）
Measured in a cell proliferation assay using B16-F1 cells. Recombinant Bovine Fetuin A stimulates adhesion of B16-F1 cells. The ED50 for this effect is ≤100 μg/mL. Optimal concentration depends on cell type as well as the application or research objectives.
电泳（SDS-PAGE）
Greater than 95% as determined by reducing SDS-PAGE.
特别提示：本公司的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用。
关键字： 科研专供;GMP级;批间一致;高纯度;
在生物科技领域，丰度蛋白专注重组蛋白供应，以“丰度的风帆为蛋白提供动力，丰度为蛋白护航”为使命，赋能生命科学发展。
苏州丰度依托先进技术平台，打造成熟生产体系，严控基因工程设计、细胞培养、蛋白纯化等环节，优化工艺与管理，确保产品高纯度、高活性且批间一致。产品覆盖基础研究、生物医药研发等多领域，为科研及生产提供多样、适配产品，助力突破技术难题 。