Flotillin-2 antibodies are essential tools in studying the structure and function of membrane microdomains, particularly lipid rafts. Flotillin-2 (also known as reggie-1) is a conserved, ubiquitously expressed protein that localizes to cholesterol-rich membrane domains and plays roles in membrane trafficking, signal transduction, and cytoskeletal organization. It forms hetero-oligomers with its homolog Flotillin-1. contributing to cellular processes like endocytosis, cell adhesion, and pathogen entry. Antibodies targeting Flotillin-2 are widely used to investigate its expression, subcellular localization, and interactions in various contexts, including cancer metastasis, neuronal development, and immune responses. These antibodies (polyclonal or monoclonal) are validated for techniques such as Western blotting, immunofluorescence, and co-immunoprecipitation. Specificity is critical due to homology with Flotillin-1 and potential cross-reactivity. Researchers also utilize Flotillin-2 antibodies to explore its association with diseases; for example, elevated Flotillin-2 levels correlate with tumor progression and poor prognosis in certain cancers. Challenges include distinguishing membrane-bound versus cytosolic pools and accounting for post-translational modifications. Overall, Flotillin-2 antibodies remain pivotal in unraveling the biological and pathological significance of lipid raft-associated proteins.