PHD1/prolylhydroxylase抗体,Rabbit Monoclonal PHD1/prolylhydroxylase Antibody
  • PHD1/prolylhydroxylase抗体,Rabbit Monoclonal PHD1/prolylhydroxylase Antibody
  • PHD1/prolylhydroxylase抗体,Rabbit Monoclonal PHD1/prolylhydroxylase Antibody
  • PHD1/prolylhydroxylase抗体,Rabbit Monoclonal PHD1/prolylhydroxylase Antibody

PHD1/prolylhydroxylase抗体—艾普蒂 新品

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 上海
更新日期 2025-05-23
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产品详情

中文名称:PHD1/prolylhydroxylase抗体英文名称:Rabbit Monoclonal PHD1/prolylhydroxylase Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 7786 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Rabbit
偶联物: 靶点: PHD1/prolylhydroxylase
2025-05-23 PHD1/prolylhydroxylase抗体 Rabbit Monoclonal PHD1/prolylhydroxylase Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 7786 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHCIHC:1/100-1/200;IHF:1/50-1/200 Human,Mouse,Rat
ICC1/50-1/200 Human,Mouse,Rat
FCM1/20-1/100 Human,Mouse,Rat
Elisa咨询技术 Human,Mouse,Rat
   

产品详情

AliasesEstrogen-induced tag 6; HPH-3;;PHD1
WB Predicted band size44 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human PHD1
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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       Western blot analysis of PHD1 in (1) HeLa cell lysate; (2) A549 cell lysate.    


           

参考文献

以下是关于PHD1/prolyl hydroxylase抗体的3篇参考文献示例,包含文献名称、作者及摘要内容概括:

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1. **文献名称**:*Role of prolyl hydroxylase domain proteins in oxygen sensing and HIF-1α regulation*

**作者**:Epstein, A.C.R. et al.

**摘要**:该研究阐明了PHD1(EGLN2)作为氧依赖性羟化酶,通过羟化HIF-1α的脯氨酸残基调控其稳定性。作者利用特异性抗体验证了PHD1在细胞内的表达定位及与HIF-1α的相互作用,证实其在缺氧信号通路中的核心作用。

2. **文献名称**:*Genetic evidence for a role of prolyl hydroxylase 1 in physiological oxygen sensing*

**作者**:Aragones, J. et al.

**摘要**:通过构建PHD1基因敲除小鼠模型,结合抗体检测组织特异性表达,发现PHD1缺失导致HIF-2α异常积累,影响血管生成和红细胞生成,揭示了PHD1在氧稳态中的生理功能。

3. **文献名称**:*Selective inhibition of hypoxia-inducible HIF prolyl hydroxylases by antibodies*

**作者**:Schofield, C.J. & Ratcliffe, P.J.

**摘要**:该文献开发了针对PHD家族(包括PHD1)的特异性抗体,并验证其抑制活性。研究发现PHD1抗体可阻断HIF-α羟化,为靶向PHD1的缺氧相关疾病治疗提供工具。

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以上文献均通过抗体实验(如免疫印迹、免疫组化)验证PHD1功能,涵盖其分子机制、生理作用及潜在应用。如需具体文献来源,建议通过PubMed或Web of Science检索标题与作者获取全文。

       

背景信息

**Background of PHD1/Prolyl Hydroxylase Antibody**

PHD1 (prolyl hydroxylase domain-containing protein 1), also known as EGLN2 or HIF-PH2. is a member of the prolyl hydroxylase family that regulates cellular oxygen sensing. It catalyzes the hydroxylation of specific proline residues on hypoxia-inducible factor alpha (HIF-α), marking it for proteasomal degradation via the von Hippel-Lindau (VHL) E3 ubiquitin ligase complex. This process is critical in maintaining oxygen homeostasis. Unlike its isoforms (PHD2 and PHD3), PHD1 exhibits distinct tissue expression patterns, with higher levels in metabolically active tissues like the liver, adipose, and testes, suggesting roles beyond HIF regulation, including fatty acid metabolism and reactive oxygen species (ROS) management.

Antibodies targeting PHD1 are essential tools for studying its expression, localization, and function in both physiological and pathological contexts. They enable detection of endogenous PHD1 in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Research applications include investigating PHD1's involvement in diseases like cancer, ischemic disorders, and metabolic syndromes. For instance, PHD1 inhibition has been explored to stabilize HIF-α in tumors or ischemic tissues, promoting angiogenesis and adaptive survival. However, cross-reactivity with other PHD isoforms remains a challenge, emphasizing the need for antibody validation. High-quality PHD1 antibodies are pivotal for elucidating its tissue-specific roles and therapeutic potential in hypoxia-related pathways.

       
关键字: PHD1/prolylhydroxyla;PHD1/prolylhydroxyla;PHD1/prolylhydroxyla;

公司简介

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主营行业 抗体,蛋白组学,细胞生物学 经营模式 贸易,工厂,试剂,定制,服务
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VIP 1年
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  • 主营产品:重组蛋白、抗体、生物试剂
  • 公司地址:青浦区
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