**Background of ENDOG Antibody**
ENDOG (Endonuclease G) is a mitochondrial protein encoded by the *ENDOG* gene, belonging to the DNA/RNA non-specific ββα-Me-finger nuclease family. Initially identified as a mitochondrial enzyme involved in apoptosis, ENDOG is released from mitochondria during cell death and participates in caspase-independent DNA fragmentation. Beyond apoptosis, studies suggest roles in maintaining mitochondrial genome stability, inflammation, and cellular responses to replication stress.
ENDOG antibodies are essential tools for detecting and studying the protein's expression, localization, and function. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate ENDOG's involvement in physiological processes (e.g., embryogenesis, germ cell development) and pathologies such as cancer, neurodegenerative diseases, and ischemia-reperfusion injury.
Commercial ENDOG antibodies are typically raised in hosts like rabbits or mice, targeting specific epitopes (e.g., human ENDOG around 28-32 kDa). Validation often includes knockout controls to confirm specificity. Recent research also explores ENDOG's non-apoptotic roles, such as regulating innate immunity and mitochondrial dynamics, expanding its relevance in biomedical research.