The PCBP4 (poly(rC)-binding protein 4) antibody is a research tool used to study the function and expression of the PCBP4 protein, a member of the poly(C)-binding protein (PCBP) family. PCBPs are RNA-binding proteins involved in post-transcriptional regulation, including mRNA stability, splicing, and translation. PCBP4. also known as αCP4. contains three K-homology (KH) domains that mediate interactions with RNA or DNA. Unlike PCBP1-3. which are ubiquitously expressed and play roles in diverse cellular processes, PCBP4 is less characterized but has been implicated in tumor suppression. Studies suggest PCBP4 is induced by DNA damage or oncogenic stress and regulates pathways like TGF-β signaling and p53-mediated apoptosis, influencing cell cycle arrest or programmed cell death. Its dysregulation is linked to cancers, including lung, liver, and colorectal malignancies, where it may act as a tumor suppressor by inhibiting proliferation or metastasis.
The PCBP4 antibody enables detection and quantification of PCBP4 in techniques like Western blotting, immunoprecipitation, and immunohistochemistry. It aids in exploring PCBP4's subcellular localization, protein interactions, and expression patterns in normal versus diseased tissues. Research using this antibody has highlighted PCBP4's potential as a biomarker or therapeutic target in cancer. However, variability in antibody specificity and validation across studies underscores the need for rigorous controls. Ongoing research aims to clarify PCBP4's molecular mechanisms and translational relevance in human diseases.