The NDUFAB1 antibody is a research tool designed to detect the NDUFAB1 protein, a critical subunit of mitochondrial Complex I (NADH:ubiquinone oxidoreductase) in the electron transport chain. NDUFAB1. also known as NADH dehydrogenase [ubiquinone] 1 alpha/beta subcomplex subunit 1. plays an essential role in Complex I assembly and function by facilitating electron transfer and contributing to structural stability. This protein is unique as it serves dual roles: acting as a subunit of Complex I and functioning independently as a mitochondrial acyl carrier protein involved in fatty acid biosynthesis.
NDUFAB1 antibodies are widely used in studies investigating mitochondrial dysfunction, which is linked to neurodegenerative disorders (e.g., Parkinson’s and Alzheimer’s diseases), metabolic syndromes, and cancers. Researchers employ these antibodies in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess NDUFAB1 expression levels, subcellular localization, and interactions in disease models or genetic knockout studies.
Most commercially available NDUFAB1 antibodies are produced in rabbits or mice using immunogenic peptide sequences specific to human NDUFAB1. with cross-reactivity tested in common model organisms. Validation typically includes verification via siRNA knockdown or CRISPR-Cas9 knockout controls to confirm specificity. Understanding NDUFAB1's role through such antibodies provides insights into cellular energy metabolism, oxidative stress mechanisms, and potential therapeutic targets for mitochondrial disorders.