The CFAP45 (Cilia and Flagella Associated Protein 45) antibody is a research tool designed to detect and study the CFAP45 protein, which plays a critical role in the structure and function of motile cilia and flagella. CFAP45 is a conserved, axonemal protein predominantly localized in the ciliary axoneme, where it contributes to the assembly, stability, and coordinated movement of these hair-like cellular projections. Dysfunction of CFAP45 has been linked to ciliopathies, such as primary ciliary dyskinesia (PCD), a genetic disorder characterized by impaired mucociliary clearance, chronic respiratory infections, and infertility.
The CFAP45 antibody is widely used in immunological techniques (e.g., Western blot, immunofluorescence, immunohistochemistry) to investigate protein expression, subcellular localization, and pathological mechanisms in disease models. It has been validated in multiple species, including humans and mice, aiding in comparative studies. Recent research highlights its utility in identifying CFAP45 mutations in PCD patients and exploring its interaction with other axonemal proteins like DNAH5 and SPEF2.
As cilia-related research expands, the CFAP45 antibody remains essential for elucidating the molecular basis of ciliary motility disorders and developing diagnostic or therapeutic strategies. Commercial antibodies are typically raised in rabbits or mice, with specificity confirmed via knockout validation.