The NUDT5 antibody is a crucial tool for studying the NUDT5 protein (nucleoside diphosphate-linked moiety X-type motif 5), a member of the Nudix hydrolase family involved in nucleotide metabolism. NUDT5 catalyzes the hydrolysis of nucleoside diphosphate derivatives, including ADP-ribose and 8-oxo-dGDP, playing roles in maintaining cellular nucleotide pools and mitigating oxidative DNA damage. It has been implicated in diverse physiological processes, such as hormone signaling, inflammation, and cancer progression, particularly in estrogen receptor-positive breast cancer where it regulates ADP-ribose metabolism.
NUDT5 antibodies are widely used in research applications like Western blotting, immunohistochemistry, and immunoprecipitation to detect protein expression, localization, and interaction partners. Their development often involves immunizing hosts with peptide antigens derived from conserved regions of human NUDT5. followed by hybridoma technology (for monoclonal antibodies) or affinity purification (for polyclonal versions). Validation typically includes knockout cell lines or siRNA-mediated silencing to confirm specificity.
Recent studies highlight NUDT5's potential as a therapeutic target, driving demand for high-affinity antibodies to explore its mechanistic roles in diseases. Commercial antibodies vary in clonality, epitope recognition, and cross-reactivity across species, necessitating careful selection based on experimental needs. Ongoing research using these reagents aims to clarify NUDT5's contribution to metabolic reprogramming in tumors and its interplay with DNA repair pathways.