The HUWE1 antibody is a crucial tool for studying the HUWE1 protein, an E3 ubiquitin ligase central to regulating cellular processes like DNA repair, apoptosis, and cell cycle control. HUWE1 (HECT, UBA, and WWE domain-containing E3 ubiquitin protein ligase 1) mediates substrate ubiquitination, targeting proteins such as p53. Myc, and Mcl-1 for proteasomal degradation, thereby influencing tumor suppression, oncogenesis, and cellular homeostasis. Dysregulation of HUWE1 is linked to cancers, neurodevelopmental disorders (e.g., intellectual disability syndromes), and neurodegenerative diseases. Researchers use HUWE1 antibodies in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to analyze its expression, localization, and interactions across tissues. These antibodies help uncover HUWE1’s dual roles—acting as both an oncogene and tumor suppressor depending on context—and its involvement in stress responses. Recent studies also explore its impact on mitochondrial function and synaptic plasticity. By enabling precise detection of HUWE1 isoforms and post-translational modifications, these antibodies are vital for dissecting molecular mechanisms in disease models and evaluating therapeutic strategies targeting ubiquitination pathways.