ZNHIT3 (Zinc Finger HIT-Type Containing 3) is a protein encoded by the ZNHIT3 gene, belonging to the zinc finger HIT-type protein family. It is a component of the SNF2-related CREBBP activator protein (SRCAP) complex, which plays a critical role in chromatin remodeling by facilitating the deposition of the histone variant H2A.Z into nucleosomes. This process is essential for regulating gene expression, DNA repair, and maintaining genomic stability. ZNHIT3 specifically interacts with the SRCAP subunit, contributing to the complex's ability to catalyze ATP-dependent nucleosome repositioning.
Antibodies targeting ZNHIT3 are valuable tools for studying its expression, localization, and functional interactions in cellular processes. They are commonly used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate ZNHIT3's role in chromatin dynamics, transcriptional regulation, and disease mechanisms. Dysregulation of ZNHIT3 has been implicated in cancers, developmental disorders, and neurological conditions, making its detection crucial for understanding pathological pathways. Commercial ZNHIT3 antibodies are typically validated for specificity using knockout cell lines or siRNA-mediated knockdown to ensure reliable experimental outcomes. Research on ZNHIT3 continues to expand, highlighting its potential as a therapeutic target or biomarker in diseases linked to epigenetic dysregulation.