Eps15 homology domain-containing protein 3 (EHD3) is a member of the EHD protein family, which plays a critical role in membrane trafficking and endocytic recycling. EHD3. along with other family members (EHD1. EHD2. and EHD4), regulates intracellular transport pathways, particularly the recycling of receptors and cargo proteins from endosomes to the Golgi apparatus or plasma membrane. EHD3 is distinguished by its preference for Rab11-positive recycling compartments and its involvement in retrograde transport. It is highly expressed in tissues with dynamic membrane trafficking needs, such as the nervous system, muscles, and cardiovascular tissues.
Antibodies targeting EHD3 are essential tools for studying its localization, expression, and functional interactions. These antibodies are commonly used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate EHD3’s role in cellular processes. For example, studies employing EHD3 antibodies have revealed its involvement in cardiac function, neuronal development, and insulin receptor trafficking. Dysregulation of EHD3 has been linked to diseases such as cancer, neurodegenerative disorders, and cardiovascular conditions, making these antibodies valuable for both basic research and clinical investigations.
EHD3 antibodies are typically validated for specificity using knockout cell lines or tissues to ensure minimal cross-reactivity with other EHD family members. Commercially available clones may vary in host species (e.g., rabbit, mouse) and epitope recognition, allowing flexibility in experimental design. Their application continues to advance understanding of endocytic pathways and their implications in health and disease.