SHMT1抗体,Mouse Monoclonal SHMT1 Antibody
  • SHMT1抗体,Mouse Monoclonal SHMT1 Antibody
  • SHMT1抗体,Mouse Monoclonal SHMT1 Antibody
  • SHMT1抗体,Mouse Monoclonal SHMT1 Antibody

SHMT1抗体;SHMT1 Antibody—艾普蒂

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 上海
更新日期 2025-05-29
QQ交谈 微信洽谈

产品详情

中文名称:SHMT1抗体英文名称:Mouse Monoclonal SHMT1 Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 7607 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Mouse
偶联物: 靶点: SHMT1
2025-05-29 SHMT1抗体 Mouse Monoclonal SHMT1 Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 7607 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF1/100-1/200 Human,Mouse,Rat
IHC1/50-1/250 Human,Mouse,Rat
ICC技术咨询 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa咨询技术 Human,Mouse,Rat
   

产品详情

Host/IsotypeMouse IgG2a
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human SHMT1
FormulationPurified antibody in PBS with 0.05% sodium azide

+ +

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       Lane 1: Jurkat lysate ;  Lane 2: SHMT1 immunoprecipitated from Jurkat lysate by P15540 ;  Lane 3: The same  Lane 2 but use  IgG isotype control antibody ;Result: P15540 can immunoprecipitate SHMT1;    


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       IHC-P analysis of kidney tissue by SHMT1 antibody (P15540) IHC-P was performed using sections of the formalin-fixed paraffin-embedded kidney tissue;Result: Cells in tubules are positively stained at the cytoplasm.    


           

参考文献

以下是关于SHMT1抗体的3篇参考文献,按文献名称、作者和摘要内容简要概括:

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1. **文献名称**:*"Serine hydroxymethyltransferase 1 knockdown induces apoptosis in cervical cancer cells through inhibition of mitochondrial biogenesis and ATP production"*

**作者**:Liu Y et al.

**摘要**:该研究通过Western blot和免疫荧光技术,使用SHMT1特异性抗体(来源:兔多克隆抗体,ABCam)验证基因沉默效果,发现SHMT1下调导致宫颈癌细胞线粒体功能受损和凋亡,提示其作为潜在治疗靶点。

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2. **文献名称**:*"Metabolic role of cytoplasmic SHMT1 in one-carbon metabolism and hepatocellular carcinoma progression"*

**作者**:Jain M et al.

**摘要**:作者利用SHMT1抗体(货号:sc-365092.小鼠单克隆抗体)进行免疫组化分析,发现SHMT1在肝癌组织中高表达,并通过调节一碳代谢促进肿瘤增殖,为代谢重编程研究提供依据。

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3. **文献名称**:*"Development and validation of a SHMT1-specific polyclonal antibody for functional studies in folate metabolism"*

**作者**:Anderson DD et al.

**摘要**:本文描述了兔源SHMT1多克隆抗体的开发流程,通过ELISA、免疫印迹和免疫沉淀验证其特异性,证明该抗体适用于检测细胞及小鼠模型中SHMT1的表达与功能,支持叶酸代谢相关研究。

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4. **文献名称**:*"SHMT1 expression correlates with poor prognosis in colorectal cancer: a tissue microarray analysis"*

**作者**:Wang H et al.

**摘要**:研究采用SHMT1抗体(兔源,Cell Signaling Technology)对结直肠癌组织芯片进行染色,结合生存数据分析,发现SHMT1高表达与患者预后不良显著相关,提示其作为生物标志物的潜力。

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这些文献涵盖了SHMT1抗体在癌症机制、代谢研究和诊断工具开发中的应用,涉及Western blot、免疫组化等技术场景。如需全文链接或补充信息,可进一步提供具体需求。

       

背景信息

SHMT1 (Serine Hydroxymethyltransferase 1) is a key metabolic enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate (THF) to glycine and 5.10-methylene-THF. This reaction is critical in one-carbon metabolism, supporting nucleotide synthesis, DNA repair, and methylation processes. SHMT1 is primarily cytosolic and highly expressed in tissues with rapid cell division, such as the liver and certain cancers, making it a focus in oncology and metabolic disease research.

SHMT1 antibodies are essential tools for detecting and quantifying SHMT1 protein levels in various experimental models. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study SHMT1's expression patterns, subcellular localization, and regulatory mechanisms. Research has linked SHMT1 dysregulation to cancer progression, folate deficiency disorders, and neural tube defects, underscoring its biomedical relevance.

Commercially available SHMT1 antibodies are typically developed in hosts like rabbits or mice, targeting specific epitopes of human or rodent SHMT1. Validation often includes knockout (KO) cell line controls to ensure specificity. These antibodies aid in exploring SHMT1's role in metabolic adaptation, drug resistance, and as a potential therapeutic target. However, researchers must verify batch consistency and optimize protocols due to variability in antibody performance across applications.

       
关键字: SHMT1抗体;SHMT1;SHMT1 Antibody;

公司简介

生物技术有限公司
成立日期 2024-07-02 (2年) 注册资本 20万人民币
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 抗体,植物生物技术,细胞培养,蛋白组学 经营模式 贸易,工厂,试剂,定制,服务
  • 上海切尔齐生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:20万人民币
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:生物试剂
  • 公司地址:青浦
询盘

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