Hemoglobinsubunitalpha抗体;Hemoglobinsubunitalpha Antibody—艾普蒂 新品

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     Western blot analysis of Hemoglobin subunit alpha expression in K562 cell lysate.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human spleen, using hemoglobin subunit alpha Antibody.    

  
  

Hemoglobin subunit alpha (Hbα) antibodies are immunological tools designed to target the alpha globin chains of hemoglobin, the oxygen-carrying protein in red blood cells. Hemoglobin consists of two alpha and two beta subunits (α2β2), each bound to a heme group. The alpha globin chains are encoded by the *HBA1* and *HBA2* genes, located on chromosome 16. Mutations in these genes can lead to alpha-thalassemia, a genetic disorder characterized by reduced alpha globin production.

Hbα antibodies are widely used in research and diagnostics to detect, quantify, and localize alpha globin in biological samples. They are essential for studying hemoglobin synthesis, erythrocyte development, and diseases like thalassemia, myelodysplastic syndromes, or hemoglobinopathies. These antibodies are typically generated in animal hosts (e.g., rabbits, mice) using recombinant alpha globin proteins or synthetic peptides as immunogens. Validation methods include Western blotting, immunohistochemistry, and ELISA to ensure specificity, often confirmed using knockout cell lines or tissues.

Commercial Hbα antibodies vary in clonality (monoclonal/polyoclonal), epitope specificity, and conjugates (e.g., fluorescent tags). Researchers must verify cross-reactivity with related subunits (e.g., Hbβ) and species compatibility. Beyond basic research, Hbα antibodies have potential clinical applications, such as diagnosing hemoglobin variants or monitoring erythropoiesis in bone marrow disorders. Quality control remains critical, as nonspecific binding can arise due to hemoglobin's high abundance and structural conservation across species.