**Background of CyclinB2 Antibody**
CyclinB2 is a regulatory protein belonging to the cyclin family, which plays a critical role in controlling cell cycle progression, particularly during the transition from the G2 phase to mitosis. As a key component of the cyclin-dependent kinase (CDK) complex, CyclinB2 binds to CDK1 to regulate the timing and fidelity of mitotic entry. Unlike CyclinB1. which primarily localizes to the cytoplasm and nucleus, CyclinB2 exhibits distinct subcellular distribution, often associated with the Golgi apparatus, suggesting potential roles in coordinating cell division with organelle dynamics.
Antibodies targeting CyclinB2 are essential tools for studying its expression, localization, and function in both normal and pathological contexts. These antibodies are typically developed in hosts such as rabbits or mice using immunogenic peptides or recombinant proteins corresponding to specific regions of CyclinB2. They are widely utilized in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate CyclinB2's involvement in cell cycle regulation, DNA damage responses, and cancer biology. Dysregulation of CyclinB2 has been implicated in tumorigenesis, with altered expression observed in malignancies such as breast and colorectal cancers.
Researchers rely on CyclinB2 antibodies to explore its interaction partners, post-translational modifications, and tissue-specific roles. Validated antibodies with high specificity are crucial for distinguishing CyclinB2 from homologous proteins (e.g., CyclinB1) and minimizing cross-reactivity. Such tools advance our understanding of cell cycle mechanisms and their therapeutic targeting in diseases characterized by uncontrolled proliferation.