CNPase抗体;CNPase Antibody—艾普蒂

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     Western blot analysis of CNPase expression in C6 cell lysate.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

CNPase (2',3'-cyclic nucleotide 3'-phosphodiesterase) is an enzyme predominantly expressed in the central nervous system, particularly within myelinating cells such as oligodendrocytes and Schwann cells. It plays a critical role in maintaining myelin integrity and is involved in the metabolism of cyclic nucleotides, though its precise biological function remains under investigation. CNPase exists as two splice variants (CNP1 and CNP2) and is localized to the inner mitochondrial membrane and cytoplasmic compartments of myelinating cells. Due to its specific expression pattern, CNPase has been widely used as a biomarker for studying oligodendrocyte differentiation, myelination processes, and demyelinating diseases like multiple sclerosis.

Antibodies targeting CNPase are essential tools in neuroscience research, enabling the identification and characterization of oligodendrocytes in both developmental and pathological contexts. These antibodies are commonly employed in techniques such as immunohistochemistry, Western blotting, and immunofluorescence to visualize myelination dynamics, assess neural injury models, or investigate disorders affecting white matter. Commercially available CNPase antibodies are typically raised against specific epitopes of human or rodent CNPase proteins, with validation in species including mice, rats, and humans. Researchers often verify antibody specificity using knockout models or siRNA-mediated knockdown. Both monoclonal and polyclonal variants exist, with monoclonal antibodies offering higher specificity and polyclonal antibodies providing broader detection across experimental conditions. Proper validation remains crucial due to potential cross-reactivity with unrelated proteins in certain tissues.