Involucrin抗体;Involucrin Antibody—艾普蒂 新品

+ +

•  

   

     Western blot analysis of Involucrin expression in A431 cell lysate.    

  
  

•  

   

     Fibulin-3 has anti-tumorigenic activities in cutaneous squamous cell carcinoma. -Journal of Investigative Dermatology    

  
  

Involucrin is a key structural protein involved in epidermal differentiation, primarily expressed in the suprabasal layers of stratified squamous epithelia, such as the skin. It plays a critical role in forming the cornified envelope, a protective barrier by cross-linking with other proteins (e.g., loricrin, filaggrin) during terminal keratinocyte differentiation. As a marker of late-stage epidermal maturation, involucrin is widely used in dermatological research to study skin homeostasis, barrier dysfunction, and diseases like psoriasis, eczema, or squamous cell carcinoma.

Involucrin antibodies are essential tools for detecting and localizing this protein in tissues or cultured cells. They are commonly employed in techniques like immunohistochemistry (IHC), immunofluorescence (IF), and Western blotting to assess epidermal differentiation status or pathological changes. These antibodies help identify aberrations in involucrin expression, which may correlate with impaired barrier function, hyperproliferative disorders, or tumor progression. Monoclonal antibodies are preferred for specificity, while polyclonal versions may offer broader epitope recognition. Validation often includes testing on knockout models or siRNA-treated cells to confirm target specificity. Cross-reactivity with involucrin orthologs in animal models (e.g., mice, rats) is critical for translational studies. Researchers also utilize these antibodies to explore therapeutic interventions targeting epidermal repair or differentiation pathways. Proper antibody selection and validation are crucial, as inconsistent results may arise from epitope masking in formalin-fixed tissues or variations in post-translational modifications across species.