Trefoil Factor 3 (TFF3) is a small secretory protein belonging to the trefoil factor family, characterized by a conserved three-loop "trefoil" domain stabilized by disulfide bonds. Primarily expressed in intestinal goblet cells and respiratory epithelia, TFF3 plays critical roles in mucosal protection, repair, and regeneration by promoting cell migration, reducing apoptosis, and modulating inflammation. It is also implicated in maintaining epithelial barrier integrity and interacting with signaling pathways such as EGFR and Wnt. Dysregulation of TFF3 has been linked to gastrointestinal cancers, inflammatory bowel disease (IBD), and chronic respiratory conditions, where its expression often correlates with disease progression or healing.
TFF3 antibodies are essential tools for detecting and quantifying TFF3 in research and diagnostics. These antibodies, typically developed in rabbits or mice, target specific epitopes of TFF3 and are used in techniques like Western blotting, immunohistochemistry (IHC), and ELISA. High-quality TFF3 antibodies exhibit high specificity and sensitivity, enabling precise localization in tissues or accurate measurement in biological fluids. Clinically, TFF3 antibodies aid in studying its role as a biomarker for gastrointestinal cancers or monitoring IBD treatment responses. Recent studies also explore TFF3's therapeutic potential, with antibodies helping to validate its mechanisms in preclinical models. Reliable TFF3 antibodies thus bridge basic research and clinical applications, offering insights into mucosal health and disease.