The VPS11 antibody is a research tool used to study the Vacuolar Protein Sorting 11 (VPS11) protein, a key component of cellular trafficking pathways. VPS11 is part of the HOPS (Homotypic Fusion and Protein Sorting) complex, which mediates lysosome biogenesis, endosome-lysosome fusion, and autophagosome maturation. It plays a critical role in regulating vesicular transport, membrane fusion, and organelle acidification. Dysfunction of VPS11 is linked to lysosomal storage disorders, neurodegenerative diseases, and cancer.
The antibody specifically targets VPS11. enabling detection and localization of the protein in cells or tissues. It is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate VPS11 expression, interaction partners, and cellular distribution. Researchers utilize this tool to explore mechanisms underlying lysosomal defects, autophagy impairment, or abnormal protein aggregation in diseases like Alzheimer's or Parkinson's.
Commercial VPS11 antibodies are typically developed in hosts like rabbits or mice, validated for specificity using knockout controls. Its applications span basic research in cell biology, drug discovery targeting lysosomal pathways, and diagnostic studies of genetic disorders. However, optimal performance may vary depending on experimental conditions, requiring careful optimization. Overall, the VPS11 antibody is essential for deciphering the protein's role in cellular homeostasis and disease pathology.