CholineAcetyltransferase抗体;CholineAcetyltransferase Antibody—艾普蒂 新品

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     Western blot analysis of Choline Acetyltransferase expression in Mouse brain cell lysate.    

  
  

Choline acetyltransferase (ChAT) is the enzyme responsible for synthesizing the neurotransmitter acetylcholine (ACh) from choline and acetyl-CoA, a critical process in cholinergic signaling. ChAT antibodies are immunological tools designed to detect and quantify the presence of this enzyme in biological samples. These antibodies are widely used in neuroscience research to identify cholinergic neurons, map their distribution in the central and peripheral nervous systems, and investigate dysregulation in neurological disorders such as Alzheimer’s disease, Parkinson’s disease, and myasthenia gravis.

ChAT antibodies are typically generated in hosts like rabbits or mice using purified recombinant ChAT protein or specific peptide sequences as immunogens. Their specificity is validated through techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF), often showing reactivity across species (human, mouse, rat). In research, they help visualize cholinergic pathways in brain sections, assess changes in ChAT expression in disease models, or confirm cholinergic phenotypes in cultured cells. Some studies also utilize ChAT antibodies to explore neuroplasticity, aging, or the effects of therapeutics targeting cholinergic systems. Due to the role of ACh in cognitive and motor functions, these antibodies remain vital for understanding cholinergic contributions to both health and disease.