Engrailed1抗体;Engrailed1 Antibody—艾普蒂 新品

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     Western blot analysis of Engrailed 1 expression in 293T cell lysate.    

  
  

The Engrailed1 (EN1) antibody is a crucial tool in developmental biology and neuroscience research, targeting the Engrailed1 protein—a homeodomain-containing transcription factor essential for embryonic patterning and neuronal development. First identified in Drosophila segmentation studies, EN1 orthologs are evolutionarily conserved across species, including mice and humans. In vertebrates, EN1 plays a pivotal role in midbrain-hindbrain boundary formation, cerebellar development, and dopaminergic neuron specification. It also regulates limb patterning and epidermal differentiation.

Researchers widely use EN1 antibodies to study neural tube defects, neurodegenerative disorders (e.g., Parkinson's disease), and cancers (notably basal cell carcinoma and medulloblastoma) where EN1 expression is dysregulated. These antibodies are typically produced in rabbit or mouse hosts using immunogens corresponding to specific EN1 epitopes (e.g., N-terminal or homeodomain regions). Validated applications include immunohistochemistry, Western blotting, and in situ hybridization controls.

Recent studies leverage EN1 antibodies to explore its emerging roles in adult tissue maintenance and cancer stem cell regulation. Cross-reactivity with EN2 (a paralog sharing ~70% amino acid similarity) requires careful experimental controls. Commercial EN1 antibodies often cite validation in knockout models to ensure specificity. As EN1 continues to be implicated in neurodevelopmental disorders and tumorigenesis, its antibody remains vital for mapping protein localization and functional interactions in developmental and disease contexts.