HistoneH3(acetylK9)抗体;HistoneH3(acetylK9) Antibody—艾普蒂 新品

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

Histone H3 acetylated at lysine 9 (H3K9ac) is a post-translational modification associated with transcriptional activation and chromatin relaxation. Acetylation of histone H3 at this specific residue neutralizes the positive charge of lysine, reducing its interaction with negatively charged DNA and promoting an open chromatin structure. This epigenetic mark is typically enriched at active gene promoters and enhancers, facilitating access for transcription factors and RNA polymerase II. The H3K9ac modification is dynamically regulated by histone acetyltransferases (e.g., GCN5. p300) and deacetylases (HDACs), linking it to cellular processes like gene expression, cell cycle progression, and DNA repair.

Antibodies targeting H3K9ac are widely used in chromatin immunoprecipitation (ChIP), immunofluorescence, and Western blotting to study epigenetic regulation in various contexts. Researchers employ these antibodies to investigate how chromatin remodeling influences development, differentiation, and disease states such as cancer or neurological disorders. For example, decreased H3K9ac levels have been observed in age-related cognitive decline, while abnormal patterns are reported in certain tumors. As a marker of active chromatin, H3K9ac antibodies also help map regulatory elements in genome-wide studies and assess the effects of HDAC inhibitors in therapeutic research. Proper validation with positive/negative controls (e.g., HDAC-treated samples) is critical due to potential cross-reactivity with other acetylated histone residues.