**Background of MCM4 Antibody**
The minichromosome maintenance complex component 4 (MCM4) is a critical protein involved in DNA replication initiation and elongation. As a member of the MCM2-7 hexameric helicase complex, MCM4 plays a vital role in unwinding DNA during the S phase of the cell cycle, ensuring faithful genome duplication. Dysregulation of MCM proteins is linked to replication stress, genomic instability, and cancer progression.
MCM4 antibodies are essential tools for studying DNA replication mechanisms, cell cycle regulation, and cancer biology. These antibodies specifically detect MCM4 expression in various experimental applications, including Western blotting, immunofluorescence, and immunohistochemistry. Elevated MCM4 levels are observed in proliferating cells, making it a biomarker for cellular proliferation and malignancy. Research has also implicated MCM4 mutations in genetic disorders, such as natural killer cell deficiency and adrenal gland dysfunction.
In cancer diagnostics, MCM4 antibodies help assess tumor aggressiveness and replication stress responses. Additionally, studies explore MCM4’s interaction with licensing factors (e.g., Cdc6. Cdt1) and checkpoint regulators (e.g., ATR, Chk1), providing insights into replication fork stability and therapeutic targeting. Overall, MCM4 antibodies are pivotal in unraveling replication-related pathologies and developing strategies to combat cancer and genetic diseases.