The NONO protein, also known as p54nrb, is a multifunctional RNA/DNA-binding protein belonging to the DBHS (Drosophila behavior human splicing) family. It plays critical roles in transcriptional regulation, RNA processing, nuclear retention of RNA, DNA repair, and circadian rhythm control. Antibodies targeting NONO/p54nrb are widely used to study its interactions with nuclear paraspeckles, spliceosomes, and transcription complexes. These antibodies enable detection of NONO's dynamic localization through techniques like immunofluorescence, immunoprecipitation (IP), and chromatin immunoprecipitation (ChIP).
Most commercial NONO antibodies are raised against conserved regions (e.g., amino acids 150-250 containing the PSP domain) in host species like rabbit or mouse. Specificity validation typically involves knockout cell lines or peptide competition assays. Researchers frequently use these antibodies to investigate NONO's partnership with proteins like SFPQ and PSPC1 in paraspeckle formation, its role in HIV-1 replication via interaction with Rev protein, and its involvement in cancer progression through transcriptional co-regulation with SIN3A/HDAC complexes.
Recent studies also employ NONO antibodies to explore its moonlighting functions in neurological disorders and DNA damage response. Caution is required when interpreting results due to potential cross-reactivity with other DBHS proteins or splice variants. Optimal performance varies between applications, necessitating protocol optimization for IP or chromatin studies.