GlutathioneSynthetase抗体;GlutathioneSynthetase Antibody—艾普蒂

+ +

•  

   

     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

•  

   

     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

•  

   

     Western blot analysis of Glutathione Synthetase expression in HeLa cell lysate.    

  
  

Glutathione synthetase (GSS) is a crucial enzyme in the glutathione (GSH) biosynthesis pathway, catalyzing the final step of GSH production by combining γ-glutamylcysteine with glycine. As the most abundant cellular antioxidant, glutathione plays a vital role in detoxification, redox homeostasis, and cellular defense against oxidative stress. Antibodies targeting glutathione synthetase are valuable tools for studying its expression, localization, and regulatory mechanisms in various biological contexts.

These antibodies are typically developed using immunogens such as recombinant GSS proteins or synthetic peptides corresponding to specific epitopes. They enable researchers to investigate GSS expression patterns across tissues, track its subcellular distribution (primarily cytoplasmic), and assess changes under pathological conditions like oxidative stress, neurodegenerative diseases, or cancer. Validated applications include Western blotting, immunohistochemistry, immunofluorescence, and ELISA. Specificity is often confirmed through knockout/knockdown controls or competitive assays.

Research using GSS antibodies has shed light on its involvement in metabolic disorders, particularly glutathione synthetase deficiency – a rare autosomal recessive condition causing hemolytic anemia and neurological impairments. Such studies contribute to understanding redox biology, drug metabolism, and therapeutic strategies targeting antioxidant systems.