Exportin5抗体;Exportin5 Antibody—艾普蒂 新品

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     Western blot analysis of Exportin 5 expression in (1) K562 cell lysate; (2) NIH/3T3 cell lysate.    

  
  

Exportin5 (XPO5) is a member of the karyopherin-β family of nuclear transport proteins, primarily responsible for mediating the export of precursor microRNAs (pre-miRNAs) and certain viral RNAs from the nucleus to the cytoplasm. This process is critical for RNA interference pathways and post-transcriptional gene regulation. Exportin5 recognizes its cargoes via a characteristic double-stranded RNA structure, binding cooperatively with RanGTP to facilitate transport through nuclear pore complexes. Dysregulation of XPO5 has been implicated in various diseases, including cancer and viral infections, due to its role in modulating miRNA maturation and RNA shuttling.

Antibodies targeting Exportin5 are essential tools for studying its expression, localization, and functional interactions. These antibodies are typically developed in model organisms (e.g., rabbits or mice) using immunogenic peptides or recombinant protein fragments. High-quality Exportin5 antibodies exhibit specificity confirmed by techniques like Western blotting, immunoprecipitation, or immunofluorescence, often validated through knockout cell lines. Researchers employ these reagents to investigate XPO5's involvement in cellular processes, such as miRNA biogenesis defects in tumors or viral hijacking mechanisms. Commercial antibodies often include validation data across applications (e.g., IHC, flow cytometry), enabling diverse experimental approaches. Proper antibody selection depends on target epitopes, species cross-reactivity, and intended methodologies, with controls crucial for interpreting results in complex biological systems.