HistoneH3(dimethylK4)抗体,Rabbit Monoclonal HistoneH3(dimethylK4) Antibody
  • HistoneH3(dimethylK4)抗体,Rabbit Monoclonal HistoneH3(dimethylK4) Antibody
  • HistoneH3(dimethylK4)抗体,Rabbit Monoclonal HistoneH3(dimethylK4) Antibody
  • HistoneH3(dimethylK4)抗体,Rabbit Monoclonal HistoneH3(dimethylK4) Antibody

HistoneH3(dimethylK4)抗体;HistoneH3(dimethylK4) Antibody—艾普蒂 新品

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 上海
更新日期 2025-05-30
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产品详情

中文名称:HistoneH3(dimethylK4)抗体英文名称:Rabbit Monoclonal HistoneH3(dimethylK4) Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 8681 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Rabbit
偶联物: 靶点: HistoneH3(dimethylK4)
2025-05-30 HistoneH3(dimethylK4)抗体 Rabbit Monoclonal HistoneH3(dimethylK4) Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 8681 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IFChIP:1/20-1/50 Human,Mouse,Rat
IHCIHC:1/100-1/200;IHF:1/50-1/200 Human,Mouse,Rat
ICC1/50-1/200 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa咨询技术 Human,Mouse,Rat
   

产品详情

AliasesHistone H3.1, Histone H3, HIST1H3A;;DiMethyl-Histone H3 (K5)
WB Predicted band size15 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human Histone H3.1 around the methylation site of K5
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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       Western blot analysis of Histone H3 (di methyl K4) expression in HeLa cell lysate.    


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       All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    


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       All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    


           

参考文献

以下是关于Histone H3(dimethylK4)抗体的3篇参考文献,简要概括如下:

1. **文献名称**:*"Selective recognition of methylated lysine 4 on histone H3 by the HP1 chromo domain"*

**作者**:Bannister, A.J., et al.

**摘要**:该研究鉴定了组蛋白H3K4甲基化(包括二甲基化)与染色质结合蛋白HP1的相互作用,揭示了H3K4me2在异染色质形成中的潜在作用,并验证了抗体在染色质免疫沉淀(ChIP)中的特异性。

2. **文献名称**:*"Active genes are tri-methylated at K4 of histone H3"*

**作者**:Santos-Rosa, H., et al.

**摘要**:通过使用H3K4me2特异性抗体,研究发现H3K4二甲基化在酵母基因启动子区富集,且与转录活性相关,为表观遗传调控基因表达的机制提供了证据。

3. **文献名称**:*"Methylation of histone H3K4 mediates association of the methylation machinery with active genes"*

**作者**:Bernstein, B.E., et al.

**摘要**:该文献利用H3K4me2抗体分析胚胎干细胞分化过程中的组蛋白修饰动态,发现H3K4me2标记在多能性基因启动子区的维持对细胞命运决定至关重要。

4. **文献名称**:*"Antibody-specific detection of dimethylated histone H3K4 in embryonic stem cells"*

**作者**:Cheng, X., & Blum, R.

**摘要**:研究优化了H3K4me2抗体的应用条件,验证其在胚胎干细胞中的特异性,并揭示了该修饰在维持干细胞自我更新中的功能。

以上文献均涉及H3K4me2抗体的应用及其在基因调控和表观遗传机制中的研究。

       

背景信息

The Histone H3 (dimethyl K4) antibody is a key tool for studying epigenetic regulation, specifically targeting the dimethylation of lysine 4 on histone H3 (H3K4me2). This post-translational modification is enriched in promoter regions of actively transcribed genes and is associated with transcriptional activation. H3K4me2 is deposited by methyltransferases such as SET1/COMPASS complexes and removed by demethylases like LSD1/KDM1A or JARID1/KDM5 family proteins. It plays a critical role in chromatin remodeling, maintaining an open chromatin state to facilitate transcription factor binding and RNA polymerase II recruitment.

Researchers use this antibody in techniques like chromatin immunoprecipitation (ChIP-seq), immunofluorescence (IF), and Western blotting (WB) to map H3K4me2 distribution and investigate its involvement in cellular processes, including differentiation, development, and disease. Aberrant H3K4me2 levels have been linked to cancers, neurological disorders, and metabolic syndromes, making the antibody valuable for both basic research and clinical studies.

Specificity validation is essential, as cross-reactivity with mono- or trimethylated H3K4 or other histone modifications can occur. Proper controls (e.g., knockout cells or peptide competition assays) are required to confirm antibody reliability. Commercial antibodies often provide validation data across multiple applications, but batch variability may affect performance. Understanding H3K4me2 dynamics through this antibody aids in deciphering gene regulatory mechanisms and identifying therapeutic targets in epigenetic diseases.

       
关键字: HistoneH3(dimethylK4;HistoneH3(dimethylK4;HistoneH3(dimethylK4;

公司简介

生物技术有限公司
成立日期 2024-07-02 (2年) 注册资本 20万人民币
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 抗体,植物生物技术,细胞培养,蛋白组学 经营模式 贸易,工厂,试剂,定制,服务
  • 上海切尔齐生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:20万人民币
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:生物试剂
  • 公司地址:青浦
询盘

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