HistoneH3(mutatedK27Met)抗体,Rabbit Monoclonal HistoneH3(mutatedK27Met) Antibody
  • HistoneH3(mutatedK27Met)抗体,Rabbit Monoclonal HistoneH3(mutatedK27Met) Antibody
  • HistoneH3(mutatedK27Met)抗体,Rabbit Monoclonal HistoneH3(mutatedK27Met) Antibody
  • HistoneH3(mutatedK27Met)抗体,Rabbit Monoclonal HistoneH3(mutatedK27Met) Antibody

HistoneH3(mutatedK27Met)抗体;HistoneH3(mutatedK27Met) Antibody—艾普蒂 新品

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 上海
更新日期 2025-05-30
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产品详情

中文名称:HistoneH3(mutatedK27Met)抗体英文名称:Rabbit Monoclonal HistoneH3(mutatedK27Met) Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 8698 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Rabbit
偶联物: 靶点: HistoneH3(mutatedK27Met)
2025-05-30 HistoneH3(mutatedK27Met)抗体 Rabbit Monoclonal HistoneH3(mutatedK27Met) Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 8698 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IFChIP:1/20-1/50 Human,Mouse,Rat
IHC咨询技术 Human,Mouse,Rat
ICC1/50-1/200 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa咨询技术 Human,Mouse,Rat
   

产品详情

AliasesHistone H3.1, Histone H3, HIST1H3A;;Histone H3 (K27M mutated)
WB Predicted band sizeCalculated MW: 15 kDa ; Observed MW: 18 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenA synthesized peptide derived from human Histone H3.1 (K27M mutated)
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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       Western blot analysis of Histone H3 (mutated K27 Met) expression in recombinant protein lysate.    


           

参考文献

以下是关于Histone H3K27M抗体的3篇参考文献摘要(作者、标题及核心内容简述):

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1. **标题**:*Histone H3K27M mutation in diffuse midline gliomas: a systematic review and meta-analysis*

**作者**:Schwartzentruber, J. et al.

**摘要**:该研究系统分析了H3K27M突变在儿童弥漫性中线胶质瘤中的临床意义,并验证了特异性抗体在肿瘤分型中的应用,强调了抗体在免疫组化诊断中的敏感性和特异性。

2. **标题**:*A monoclonal antibody for the detection of H3K27M mutation in pediatric gliomas*

**作者**:Bechet, D. et al.

**摘要**:报道了一种新型H3K27M单克隆抗体的开发与验证,通过免疫印迹和免疫组织化学证明其特异性,并成功应用于临床样本的突变检测。

3. **标题**:*Immunohistochemical analysis of H3K27M mutation in 947 central nervous system tumors*

**作者**:Korshunov, A. et al.

**摘要**:大规模研究验证了H3K27M抗体在多种中枢神经系统肿瘤中的诊断价值,发现其在区分弥漫性中线胶质瘤与其他肿瘤类型中的高准确性。

4. **标题**:*H3K27M mutation induces oncogenic reprogramming of chromatin architecture in gliomas*

**作者**:Chan, K.M. et al.

**摘要**:利用H3K27M特异性抗体探究突变对表观遗传调控的影响,揭示了该突变通过改变组蛋白修饰谱驱动肿瘤发生的机制。

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以上文献均聚焦于H3K27M抗体的开发、验证及在肿瘤研究中的应用,部分研究涉及临床诊断和分子机制探索。如需具体文献链接或补充信息可进一步说明。

       

背景信息

The Histone H3K27M mutation, where lysine 27 is replaced by methionine (K27M), is a hallmark of certain aggressive cancers, particularly diffuse midline gliomas (DMGs) and diffuse intrinsic pontine gliomas (DIPGs) in children. This oncohistone mutation disrupts epigenetic regulation by inhibiting the polycomb repressive complex 2 (PRC2), which catalyzes trimethylation of histone H3 at lysine 27 (H3K27me3), a mark associated with transcriptional silencing. The H3K27M mutation causes global reduction of H3K27me3. leading to aberrant gene expression and tumorigenesis.

Antibodies specific for Histone H3K27M are critical tools for detecting this mutation in research and diagnostics. They enable precise identification of H3K27M-altered tumors through techniques like immunohistochemistry (IHC) or Western blot, aiding in tumor classification and prognostication. These antibodies are designed to recognize the unique epitope created by the K27M substitution, ensuring specificity over wild-type H3. Their development has been pivotal in advancing understanding of H3K27M-driven oncogenesis and evaluating therapeutic strategies targeting epigenetic dysregulation. Clinically, H3K27M detection serves as a diagnostic biomarker, as the mutation is now integrated into the WHO classification of central nervous system tumors. Researchers also use these antibodies to study the mutation's role in altering chromatin structure, cellular differentiation, and tumor progression in preclinical models.

       
关键字: HistoneH3(mutatedK27;HistoneH3(mutatedK27;HistoneH3(mutatedK27;

公司简介

生物技术有限公司
成立日期 2024-07-02 (2年) 注册资本 20万人民币
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 抗体,植物生物技术,细胞培养,蛋白组学 经营模式 贸易,工厂,试剂,定制,服务
  • 上海切尔齐生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:20万人民币
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:生物试剂
  • 公司地址:青浦
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HistoneH3(mutatedK27Met)抗体;HistoneH3(mutatedK27Met) Antibody—艾普蒂相关厂家报价

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2025-05-23
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