The PEX19 antibody is a crucial tool in studying peroxisome biogenesis and related disorders. PEX19. a 299-amino acid protein, functions as a cytosolic chaperone and import receptor essential for peroxisomal membrane protein (PMP) targeting and membrane assembly. It binds nascent PMPs via conserved motifs, facilitating their delivery to peroxisomes through interaction with PEX3 on the organelle membrane. Dysfunctional PEX19 disrupts peroxisome formation, leading to severe metabolic disorders such as Zellweger spectrum disorders (ZSDs), characterized by neurological impairments and multisystem dysfunction. Antibodies targeting PEX19 enable researchers to detect its expression, localization, and interactions in cellular models. They are widely used in techniques like Western blotting, immunofluorescence, and co-immunoprecipitation to investigate peroxisome biogenesis mechanisms, disease pathogenesis, and potential therapeutic strategies. Commercially available PEX19 antibodies are typically raised in rabbits or mice, with validation in human, mouse, and rat samples. Recent studies also explore PEX19's role beyond peroxisomes, including lipid metabolism and viral infection pathways, expanding the antibody's research applications. Proper validation using knockout controls remains critical due to potential cross-reactivity with structurally similar chaperones.