Phospho-Tyrosine抗体,Mouse Monoclonal Phospho-Tyrosine Antibody
  • Phospho-Tyrosine抗体,Mouse Monoclonal Phospho-Tyrosine Antibody
  • Phospho-Tyrosine抗体,Mouse Monoclonal Phospho-Tyrosine Antibody
  • Phospho-Tyrosine抗体,Mouse Monoclonal Phospho-Tyrosine Antibody

Phospho-Tyrosine抗体;Phospho-Tyrosine Antibody—艾普蒂 新品

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 上海
更新日期 2025-06-03
QQ交谈 微信洽谈

产品详情

中文名称:Phospho-Tyrosine抗体英文名称:Mouse Monoclonal Phospho-Tyrosine Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 9011 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Mouse
偶联物: 靶点: Phospho-Tyrosine
2025-06-03 Phospho-Tyrosine抗体 Mouse Monoclonal Phospho-Tyrosine Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 9011 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB咨询技术 Transfected
IF咨询技术 Transfected
IHC1/50-1/100 Transfected
ICC技术咨询 Transfected
FCM咨询技术 Transfected
Elisa咨询技术 Transfected
   

产品详情

AliasesPhospho-Tyrosine
clone8B2
WB Predicted band sizeRefer to figures
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityTransfected
ImmunogenPurified Protein
FormulationPurified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol.

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       Western blot analysis of Phospho-Tyrosine (6G6)in Jurkat, 293T, rat Brain, mouse Brain lysates using Phospho-tyrosine antibody.    


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       Immunohistochemistry analysis of paraffin-embedded Human Breast Caricnoma using Phospho-tyrosine antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.    


           

参考文献

以下是关于Phospho-Tyrosine抗体的3-4篇参考文献,按文献名称、作者和摘要内容分类整理:

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1. **文献名称**:*"Production of antibodies to phosphotyrosine and their use for affinity purification of phosphorylated proteins"*

**作者**:Gill, G.N., et al.

**摘要**:该研究首次报道了多克隆抗磷酸酪氨酸抗体的制备,并利用这些抗体从病毒转化细胞中分离出磷酸化酪氨酸蛋白(如src激酶底物),为后续信号转导研究提供了关键工具。

2. **文献名称**:*"Identification of multiple phosphorylated tyrosine residues in polyomavirus middle tumor antigen by monoclonal antibodies"*

**作者**:Kamps, M.P., Sefton, B.M.

**摘要**:研究者开发了单克隆抗磷酸酪氨酸抗体,成功检测到多瘤病毒中T抗原的酪氨酸磷酸化位点,验证了抗体在致癌蛋白磷酸化分析中的高特异性和应用潜力。

3. **文献名称**:*"Structural and mechanistic insights into tyrosine kinase activation"*

**作者**:Hubbard, S.R., Till, J.H.

**摘要**:这篇综述总结了抗磷酸酪氨酸抗体在研究酪氨酸激酶激活机制中的作用,尤其是在解析受体酪氨酸激酶(如EGFR)的磷酸化状态及信号传递中的关键应用。

4. **文献名称**:*"Antibody-based detection of protein phosphorylation states to guide cancer therapy"*

**作者**:Blanquin, C., et al.

**摘要**:研究探讨了抗磷酸酪氨酸抗体在癌症治疗中的应用,例如通过检测PD-1/PD-L1通路的磷酸化状态指导免疫检查点抑制剂的开发,凸显其临床转化价值。

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以上文献涵盖了抗体的开发、验证及在基础研究与临床中的应用,反映了Phospho-Tyrosine抗体在信号转导和疾病机制研究中的重要性。

       

背景信息

Phospho-tyrosine (pTyr) antibodies are essential tools in molecular and cellular biology for studying protein tyrosine phosphorylation, a critical post-translational modification regulating signal transduction pathways. Tyrosine phosphorylation, mediated by kinases and reversed by phosphatases, controls diverse cellular processes such as proliferation, differentiation, and apoptosis. Dysregulation of this process is implicated in cancers, immune disorders, and neurodegenerative diseases.

The development of pTyr antibodies in the 1980s revolutionized research by enabling specific detection of phosphorylated tyrosine residues. These antibodies are typically generated by immunizing animals with phosphorylated tyrosine-containing peptides or proteins, followed by hybridoma (monoclonal) or serum-based (polyclonal) production. Monoclonal antibodies (e.g., clone 4G10. PY20) are widely used due to their high specificity.

pTyr antibodies are employed in techniques like Western blotting, immunoprecipitation, and immunofluorescence to map phosphorylation events, identify signaling intermediates, and assess kinase/phosphatase activity. They have been pivotal in characterizing oncogenic tyrosine kinases (e.g., EGFR, BCR-ABL) and validating therapeutic targets. However, challenges remain, including cross-reactivity with non-target phospho-epitopes and variability due to phosphorylation dynamics.

Their applications extend to drug discovery, biomarker validation, and diagnostics, particularly in cancer (e.g., HER2/neu testing). Ongoing advancements in antibody engineering and phosphoproteomics continue to enhance their specificity and utility in decoding complex signaling networks.

       
关键字: Phospho-Tyrosine抗体;Phospho-Tyrosine;Phospho-Tyrosine Ant;

公司简介

生物技术有限公司
成立日期 2024-07-02 (2年) 注册资本 20万人民币
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 抗体,植物生物技术,细胞培养,蛋白组学 经营模式 贸易,工厂,试剂,定制,服务
  • 上海切尔齐生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:20万人民币
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:生物试剂
  • 公司地址:青浦
询盘

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