Phospho-VEGF Receptor 2 (Tyr1175)抗体;Phospho-VEGF Receptor 2 (Tyr1175) Antibody—艾普蒂 新品

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     All lanes : Anti-RAB3B Antibody at 1:2000 dilution Lane 1: mouse brain lysate Lane 2: rat brain lysate Lane 3: SW480 whole cell lysate Lane 4: human brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

  
  

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     Overlay histogram showing HepG2 cells stained with P33313 (green line).   The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.   The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P33313,   1:25 dilution) for 60 min at 37ºC.   The secondary antibody used was Goat-Anti-Mouse IgG,  DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37ºC.   Isotype control antibody (blue line) was mouse IgG (1μg/1x10^6 cells) used under the same conditions.   Acquisition of >10,  000 events was performed.    

  
  

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     P33313 staining RAB3B in human pancreas sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed,  paraffin-embedded sections).  Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6).  Samples were incubated with primary antibody (1/25) for 1 hours at 37°C.  A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.    

  
  

Phospho-VEGF Receptor 2 (Tyr1175) antibody is a specialized tool used to detect the activated form of vascular endothelial growth factor receptor 2 (VEGFR2), a key regulator of angiogenesis and vascular development. VEGFR2. also known as KDR/Flk-1. is a receptor tyrosine kinase that binds VEGF ligands, triggering dimerization, autophosphorylation, and downstream signaling. The phosphorylation of tyrosine residue 1175 (Tyr1175) in its intracellular domain is critical for signal transduction. This site serves as a docking platform for adaptor proteins like PLCγ, initiating cascades such as MAPK and PI3K/Akt pathways, which drive endothelial cell proliferation, migration, and survival.

The antibody specifically recognizes VEGFR2 when phosphorylated at Tyr1175. enabling researchers to study receptor activation status in physiological or pathological contexts. It is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate VEGF-driven processes in cancer, diabetic retinopathy, or cardiovascular diseases. Dysregulation of VEGFR2 phosphorylation is linked to tumor angiogenesis and resistance to anti-VEGF therapies, making this antibody a valuable tool for both basic research and drug development. Its specificity helps clarify mechanisms underlying vascular pathologies and therapeutic responses, supporting advances in targeted treatments.