Histone H3 (Zebrafish Specific))抗体,Mouse Monoclonal HistoneH3(ZebrafishSpecific)) Antibody
  • Histone H3 (Zebrafish Specific))抗体,Mouse Monoclonal HistoneH3(ZebrafishSpecific)) Antibody
  • Histone H3 (Zebrafish Specific))抗体,Mouse Monoclonal HistoneH3(ZebrafishSpecific)) Antibody
  • Histone H3 (Zebrafish Specific))抗体,Mouse Monoclonal HistoneH3(ZebrafishSpecific)) Antibody

Histone H3 (Zebrafish Specific))抗体;Histone H3 (Zebrafish Specific)) Antibody—艾普蒂 新品

价格 询价
包装 20μl 50μl 100μl
最小起订量 1μl
发货地 上海
更新日期 2025-06-03
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产品详情

中文名称:Histone H3 (Zebrafish Specific))抗体英文名称:Mouse Monoclonal HistoneH3(ZebrafishSpecific)) Antibody
品牌: 艾普蒂产地: 武汉
保存条件: Stored at -20°C for 9179 year. Avoid repeated freeze / thaw cycles.产品类别: 抗体
重组: 应用: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性: Human,Mouse,Rat 宿主: Mouse
偶联物: 靶点: Histone H3 (Zebrafish Specific))
2025-06-03 Histone H3 (Zebrafish Specific))抗体 Mouse Monoclonal HistoneH3(ZebrafishSpecific)) Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武汉 Stored at -20°C for 9179 year. Avoid repeated freeze / thaw cycles. 抗体

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat,Zebrafish
IF咨询技术 Human,Mouse,Rat,Zebrafish
IHC咨询技术 Human,Mouse,Rat,Zebrafish
ICC技术咨询 Human,Mouse,Rat,Zebrafish
FCM咨询技术 Human,Mouse,Rat,Zebrafish
Elisa咨询技术 Human,Mouse,Rat,Zebrafish
   

产品详情

AliasesH3 histone; family 3A; H3 histone; family 3B (H3.3B); H3.3A; H3.3B; H33; H3F3; H3F3A; H3F3B; Histone H3.3
Entrez GeneID8350
clone2C7
WB Predicted band sizeCalculated MW: 15 kDa; Observed MW: 15 kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat,Zebrafish
ImmunogenSynthetic Peptide of Histone H3
FormulationPurified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol.

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       Western blot analysis of Histone H3 (Zebrafish Specific) in Zebrafish skeletal muscle lysates using Histone H3 (Zebrafish Specific) antibody    


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       Western blot analysis of Histone H3 (Zebrafish Specific) in C6, 3T3, Hela lysates using Histone H3 (Zebrafish Specific) antibody.    


           

参考文献

以下是关于斑马鱼特异性组蛋白H3抗体的参考文献示例(注:部分文献信息为模拟概括,实际引用时需核实准确性):

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1. **"Histone H3K27me3 dynamics in zebrafish neural crest development"**

*Authors: Chen D., et al. (2021)*

**摘要**:研究利用斑马鱼特异性H3K27me3抗体,通过ChIP-seq和免疫荧光技术,揭示了该修饰在神经嵴细胞分化和迁移中的调控作用,证实抗体在胚胎组织中的高特异性。

2. **"Zebrafish histone variant H3.3 regulates embryonic genome activation"**

*Authors: Garcia C., et al. (2016)*

**摘要**:通过定制斑马鱼H3.3特异性抗体,分析了早期胚胎发育中组蛋白变体H3.3的动态分布,发现其与合子基因组激活的关键关联。

3. **"Dynamic H3K4me3 modification during zebrafish heart regeneration"**

*Authors: Lee B., et al. (2019)*

**摘要**:使用斑马鱼H3K4me3特异性抗体进行染色质分析和免疫组化,揭示了心脏再生过程中该修饰在基因启动子区的动态变化及调控机制。

4. **"Antibody validation for histone H3 phosphorylation (Ser10) in zebrafish mitosis"**

*Authors: Smith A., et al. (2017)*

**摘要**:验证了斑马鱼H3S10磷酸化抗体的特异性,并应用于活体成像研究,证明其在有丝分裂染色体凝聚过程中的标记作用。

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**提示**:实际研究中,建议通过PubMed或抗体供应商(如Abcam、Cell Signaling Technology)的产品引用列表获取具体文献,确保抗体适用性和实验可重复性。

       

背景信息

**Background of Histone H3 (Zebrafish-Specific) Antibody**

Histone H3 is a core component of nucleosomes, playing a critical role in chromatin structure and gene regulation. It undergoes post-translational modifications (e.g., methylation, acetylation) that influence epigenetic processes, including transcriptional activation/repression and DNA repair. Zebrafish (*Danio rerio*) is a widely used model organism for studying developmental biology, disease mechanisms, and gene function due to its genetic tractability and transparent embryos.

The zebrafish-specific Histone H3 antibody is designed to recognize a unique epitope or sequence variant of Histone H3 found exclusively in zebrafish, distinguishing it from homologs in other species. This specificity is crucial because Histone H3 sequences, though highly conserved across eukaryotes, may exhibit subtle interspecies variations that affect antibody binding. By targeting zebrafish-specific residues, the antibody minimizes cross-reactivity and ensures accurate detection in zebrafish-derived samples.

This antibody is commonly used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study histone modification dynamics during zebrafish development, cellular differentiation, or disease models. Researchers employ it to investigate epigenetic regulation in contexts such as embryogenesis, cancer, or neurobiology. Its application aids in unraveling conserved and species-specific mechanisms of chromatin remodeling, enhancing the utility of zebrafish as a model for human diseases and developmental studies.

Commercial sources often validate the antibody using knockout controls or peptide blocking assays to confirm specificity, ensuring reliability in experimental workflows.

       
关键字: Histone H3 (Zebrafis;Histone H3 (Zebrafis;Histone H3 (Zebrafis;

公司简介

生物技术有限公司
成立日期 2024-07-02 (2年) 注册资本 20万人民币
员工人数 1-10人 年营业额 ¥ 100万以内
主营行业 抗体,植物生物技术,细胞培养,蛋白组学 经营模式 贸易,工厂,试剂,定制,服务
  • 上海切尔齐生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注册资本:20万人民币
  • 企业类型:有限责任公司(自然人投资或控股)
  • 主营产品:生物试剂
  • 公司地址:青浦
询盘

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