Streptavidin Protein-HRP, Horseradish peroxidase conjugated Streptavidin
分子别名(Synonym)
Streptavidin,SA
表达区间及表达系统(Source)
Streptavidin is expressed from E. coli cells and conjugated with horseradish peroxidase under optimal conditions.
蛋白结构(Molecular Characterization)
This protein carries no "tag".
The protein has a calculated MW of 13.8 kDa.
应用说明(Application)
Recommended for use in ELISA (0.1 µg/mL), immunohistochemistry, and western blot applications.
Avoid using biotin-containing solutions as diluents and solutions containing sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the reagent be titrated for optimal performance for each application.
NOTE: Do not use skim milk as a blocking agent in the assay with streptavidin, since skim milk contains free biotin which will cause high backgrounds.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 2 years in lyophilized state;
-70°C for 12 months under sterile conditions after reconstitution.


背景介绍
链霉亲和素(Streptavidin)是一种从阿维丁链霉菌中纯化的66 kDa四聚体蛋白,对生物素具有高结合亲和力,每个亚基可结合一个生物素分子。辣根过氧化物酶(HRP)是一种44 kDa糖蛋白类金属酶,经底物孵育后能产生可检测定量的显色、荧光或发光衍生物。HRP标记的链霉亲和素偶联物广泛应用于生物素标记蛋白的检测与定量分析。
关键字: Streptavidin;Streptavidin蛋白;链霉亲和素;ACRO;百普赛斯;
百普赛斯集团ACROBiosystems Group(股票代码:301080)是成立于2010年的跨国生物科技公司,是为全球生物医药、健康产业领域提供关键生物试剂产品及解决方案的行业平台型基石企业。2021年在创业板上市。百普赛斯集团业务遍布全球,横跨亚洲、北美洲、欧洲,在中国、美国、瑞士等12个城市设有办公室、研发中心及生产基地。目前累计服务客户超6000家,与全球Top 20医药企业均建立了长期、稳定的合作伙伴关系。集团旗下拥有品牌ACROBiosystems百普赛斯、bioSeedin柏思荟、Condense Capital垦拓资本和ACRODiagnostics百斯医学等。