Biotinylated Human IL-23 alpha&IL-12 beta Heterodimer Protein, His,Avitag™&Tag Free (MALS verified)
分子别名（Synonym）
IL-23 alpha & IL-12 beta
表达区间及表达系统（Source）
Biotinylated Human IL-23 alpha&IL-12 beta Heterodimer Protein, His,Avitag&Tag Free (ILB-H82W6) is expressed from human 293 cells (HEK293). It contains AA Arg 20 - Pro 189 (IL23A) & Ile 23 - Ser 328 (IL12B) (Accession # Q9NPF7-1 (IL23A) & P29460-1 (IL12B)).
Predicted N-terminus: His (IL23A) & Ile 23 (IL12B)
蛋白结构（Molecular Characterization）
Biotinylated Human IL-23A&IL-12B Heterodimer Protein, His,Avitag&Tag Free, produced by co-expression of IL23A and IL12B, has a calculated MW of 21.9 kDa (IL23A) & 34.7 kDa (IL12B). Subunit IL23A is fused with an Avi tag (Avitag™), followed by a polyhistidine tag at the N-terminus and subunit IL12B contains no tag. The protein migrates as 23-25 kDa (IL23A) & 37-39 kDa (IL12B) when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景介绍
白细胞介素-23 α亚基（IL-23 alpha）可与IL12B结合形成IL-23白细胞介素，这是一种在先天性和适应性免疫中发挥作用的异二聚体细胞因子。在外周组织中，IL-23可能与IL-17共同构成对感染的急性反应机制。IL-23通过与IL12RB1和IL23R组成的异二聚体受体复合物结合，激活Jak-Stat信号级联反应，特异性刺激记忆T细胞（而非初始T细胞），并促进促炎细胞因子的产生。该细胞因子可引发自身免疫性炎症，因此可能是自身免疫性炎症疾病的致病因子，并可能参与肿瘤发生过程。
关键字： IL23A & IL12B;IL23A蛋白;IL12B重组蛋白;ACRO;百普赛斯;
百普赛斯集团ACROBiosystems Group（股票代码：301080）是成立于2010年的跨国生物科技公司，是为全球生物医药、健康产业领域提供关键生物试剂产品及解决方案的行业平台型基石企业。2021年在创业板上市。百普赛斯集团业务遍布全球，横跨亚洲、北美洲、欧洲，在中国、美国、瑞士等12个城市设有办公室、研发中心及生产基地。目前累计服务客户超6000家，与全球Top 20医药企业均建立了长期、稳定的合作伙伴关系。集团旗下拥有品牌ACROBiosystems百普赛斯、bioSeedin柏思荟、Condense Capital垦拓资本和ACRODiagnostics百斯医学等。